Generational conservation of composition and diversity of field-acquired midgut microbiota in Anopheles gambiae (sensu lato) during colonization in the laboratory

• Published in: Parasites & vectors Vol. 12; no. 1; p. 27
• Main Authors: Akorli, Jewelna, Namaali, Philomena Asor, Ametsi, Godwin Williams, Egyirifa, Richardson Kwesi, Pels, Nana Adjoa Praba
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 11.01.2019; BioMed Central; BMC
• Subjects: Adults; Analysis; Anopheles; Anopheles gambiae; Anopheles gambiae (sensu lato); Aquatic insects; Bacteria; bacterial communities; Breeding; Breeding habitat; Clustering; Colonies; Colonization; Composition; Conservation; Culicidae; Dechlorination; Deoxyribonucleic acid; DNA; DNA sequencing; Drinking water; Eggs; females; Field water; Habitats; Instars; Intestinal flora; Intestinal microflora; intestinal microorganisms; Laboratories; Laboratory colonization; laboratory rearing; Larvae; Life history; metagenomics; Methods; Microbiota; Microbiota (Symbiotic organisms); Midgut; Midgut microbiota; mosquito vectors; Mosquitoes; Multiculturalism & pluralism; Nucleotide sequence; Parasites; Phylogenetics; Pupae; Studies; tap water; Taxonomy; Water; Water use; Field water; Breeding habitat; Laboratory colonization; Midgut microbiota; Anopheles gambiae (sensu lato)
• ISSN: 1756-3305; 1756-3305
• DOI: 10.1186/s13071-019-3287-0

The gut microbiota is known to play a role in a mosquito vector's life history, a subject of increasing research. Laboratory experiments are essential for such studies and require laboratory colonies. In this study, the conservation of field-obtained midgut microbiota was evaluated in laboratory-reared Anopheles gambiae (s.l.) mosquitoes continuously hatched in water from field breeding habitats. Pupae and late instars were obtained from the field and reared, and the emerged adults were blood-fed. The eggs obtained from them were hatched in either water from the field or in dechlorinated tap water. The mosquito colonies were maintained for 10 generations. Midguts of female adults from unfed F (emerging from field-caught pupae and larvae), F and F were dissected out and genomic DNA was extracted for 16S metagenomic sequencing. The sequences were compared to investigate the diversity and bacterial compositional differences using ANCOM and correlation clustering methods. Less than 10% of the bacterial families identified had differential relative abundances between generational groups and accounted for 46% of the variation observed. Although diversity reduced in F mosquitoes during laboratory colonization (Shannon-Weaver; P-value < 0.05), 50% of bacterial genera were conserved in those bred continuously in field-water compared to 38% in those bred in dechlorinated tap water. To our knowledge, this study is the first report on the assessment of gut bacterial community of mosquitoes during laboratory colonization and recommends the use of water from the natural breeding habitats if they are intended for microbiota research.