Characterization of Bioactive Actinomycetes Isolated from Kadolkele Mangrove Sediments, Sri Lanka

• Published in: Polish journal of microbiology Vol. 71; no. 2; pp. 191 - 204
• Main Authors: Naligama, Kishani N, Weerasinghe, Kavindi E, Halmillawewa, Anupama P
• Format: Journal Article
• Language: English
• Published: Poland De Gruyter Poland 11.06.2022; Sciendo
• Subjects: Actinomycetes; Amylases; Antibacterial activity; Bacillus cereus; Bacteria; bacterial pigments; bioactive actinomycetes; Biological activity; Biotechnology; Casein; Cellulase; E coli; Enzymes; Escherichia coli; Extracellular enzymes; Fabrics; Gene sequencing; Lagoons; Lipase; Listeria monocytogenes; mangrove sediment bacteria; Metabolites; Microorganisms; Original Paper; Pigments; Prokaryotes; Protease; rRNA 16S; Secondary metabolites; Sediments; Staphylococcus aureus; Strains (organisms); Streptomyces; Water treatment; Sri Lanka; bioactive actinomycetes; extracellular enzymes; bacterial pigments; mangrove sediment bacteria
• ISSN: 2544-4646; 1733-1331; 2544-4646
• DOI: 10.33073/pjm-2022-017

Exploring untapped microbial potentials in previously uncharted environments has become crucial in discovering novel secondary metabolites and enzymes for biotechnological applications. Among prokaryotes, actinomycetes are well recognized for producing a vast range of secondary metabolites and extracellular enzymes. In the present study, we have used surface sediments from 'Kadolkele' mangrove ecosystem located in the Negombo lagoon area, Sri Lanka, to isolate actinomycetes with bioactive potentials. A total of six actinomycetes were isolated on modified-starch casein agar and characterized. The isolates were evaluated for their antibacterial activity against four selected bacterial strains and to produce extracellular enzymes: cellulase, amylase, protease, and lipase. Three out of the six isolates exhibited antibacterial activity against , , and , but not against . Five strains could produce extracellular cellulase, while all six isolates exhibited amylase activity. Only three of the six isolates were positive for protease and lipase assays separately. Ac-1, Ac-2, and Ac-9, identified as spp. with the 16S rRNA gene sequencing, were used for pigment extraction using four different solvents. Acetone-extracted crude pigments of Ac-1 and Ac-2 were further used in well-diffusion assays, and growth inhibition of test bacteria was observed only with the crude pigment extract of Ac-2. Further, six different commercially available fabrics were dyed with crude pigments of Ac-1. The dyed fabrics retained the yellow color after acid, alkaline, and cold-water treatments suggesting the potential of the Ac-1 pigment to be used in biotechnological applications.