A Robust Single Primate Neuroepithelial Cell Clonal Expansion System for Neural Tube Development and Disease Studies

• Published in: Stem cell reports Vol. 6; no. 2; pp. 228 - 242
• Main Authors: Zhu, Xiaoqing, Li, Bo, Ai, Zongyong, Xiang, Zheng, Zhang, Kunshang, Qiu, Xiaoyan, Chen, Yongchang, Li, Yuemin, Rizak, Joshua D., Niu, Yuyu, Hu, Xintian, Sun, Yi Eve, Ji, Weizhi, Li, Tianqing
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 09.02.2016; Elsevier
• Subjects: Animals; Animals, Newborn; Cell Culture Techniques - methods; Cell Lineage; Cell Proliferation; Clone Cells; Disease Models, Animal; Folic Acid Deficiency - pathology; Macaca mulatta; Neural Tube - embryology; Neural Tube - pathology; Neural Tube Defects - pathology; Neuroepithelial Cells - cytology; Neuroglia - cytology; Neurons - cytology; Stem Cells - cytology; Stem Cells - metabolism; Telencephalon - cytology; Wnt Signaling Pathway
• ISSN: 2213-6711; 2213-6711
• DOI: 10.1016/j.stemcr.2015.10.007

Developing a model of primate neural tube (NT) development is important to promote many NT disorder studies in model organisms. Here, we report a robust and stable system to allow for clonal expansion of single monkey neuroepithelial stem cells (NESCs) to develop into miniature NT-like structures. Single NESCs can produce functional neurons in vitro, survive, and extensively regenerate neuron axons in monkey brain. NT formation and NESC maintenance depend on high metabolism activity and Wnt signaling. NESCs are regionally restricted to a telencephalic fate. Moreover, single NESCs can turn into radial glial progenitors (RGPCs). The transition is accurately regulated by Wnt signaling through regulation of Notch signaling and adhesion molecules. Finally, using the “NESC-TO-NTs” system, we model the functions of folic acid (FA) on NT closure and demonstrate that FA can regulate multiple mechanisms to prevent NT defects. Our system is ideal for studying NT development and diseases. [Display omitted] •Long-term cultured neuroepithelial stem cells (NESCs) can be induced from monkey ESCs•Single NESCs can self-organize into miniature neural tube (NT) structures•NESCs have high metabolism activity and are restricted to a telencephalic fate•The “NESC-TO-NTs” system can model and study RPGC transition and NT defect disease Li, Ji, and colleagues develop a robust and stable system to allow for clonal expansion of single monkey neuroepithelial stem cells (NESCs) to develop into miniature NT-like structures, providing a tractable in vitro platform with which to model and study radial glial progenitor transition, neural tube development, and neural tube defects.