Comparison of glycerol, lactamide, acetamide and dimethylsulfoxide as cryoprotectants of Japanese white rabbit spermatozoa

• Published in: Journal of Reproduction and Development Vol. 52; no. 4; pp. 511 - 516
• Main Authors: Kashiwazaki, N.(Azabu Univ., Sagamihara, Kanagawa (Japan). School of Veterinary Medicine), Okuda, Y, Seita,Y, Hisamitsu, S, Sonoki, S, Shino, M, Masaoka, T, Inomata, T
• Format: Journal Article
• Language: English
• Published: Japan THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 01.08.2006
• Subjects: Acetamide; Acetamides - pharmacology; Amides - pharmacology; Animals; CONEJO (ORYCTOLAGUS); CRIOPROTECTORES; Cryopreservation; Cryopreservation - instrumentation; Cryopreservation - methods; CRYOPROTECTANTS; CRYOPROTECTEUR; Cryoprotective Agents - pharmacology; Dimethyl Sulfoxide - pharmacology; ESPERMATOZOO; Female; GLICEROL; GLYCEROL; Glycerol - chemistry; Glycerol - pharmacology; Lactamide; LAPIN (ORYCTOLAGUS); Male; Rabbit spermatozoa; RABBITS; Semen Preservation - instrumentation; Semen Preservation - methods; Sperm Motility - drug effects; SPERMATOZOA; Spermatozoa - drug effects; Spermatozoa - metabolism; SPERMATOZOIDE
• ISSN: 0916-8818; 1348-4400
• DOI: 10.1262/jrd.18008

The rabbit is considered to be a valuable laboratory animal. We compared glycerol, lactamide, acetamide, and dimethylsulfoxide (DMSO) as cryoprotectants in egg-yolk diluent of ejaculated Japanese white rabbit spermatozoa for improvement of sperm cryopreservation methods. Rabbit semen was frozen with 1.0 M glycerol, lactamide, acetamide, or DMSO in plastic straws. Forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rate of forward progressive motile spermatozoa in lactamide (37.8 +- 3.0%) was significantly (P0.05) higher than in glycerol (17.0 +- 3.3%). In addition, the rates of sperm plasma membrane integrity in lactamide and acetamide (35.9 +- 3.3% and 30.2 +- 3.0%, respectively) were significantly (P0.05) higher than in glycerol (17.0 +- 2.6%). The results indicate that 1.0 M lactamide and acetamide have higher cryoprotective effects than 1.0 M glycerol for cryopreservation of Japanese white rabbit spermatozoa.