2株耐高温野生柱状田头菇的鉴定及系统发育地位研究

为了鉴定2株从四川省成都市法国梧桐树干上采集到的耐高温野生大型真菌,为其开发利用与驯化栽培奠定基础。在形态学特征观察的同时提取了子实体基因组总DNA,并对其模板进行ITS序列的扩增、测序及系统发育地位分析,并将形态学特征和系统发育地位结合来鉴定其准确分类。结果表明：从形态学上可初步鉴定2个菌株均为伞菌目（Agaricales）球盖菇科（Strophariaceae）田头菇属（Agrocybe）大型真菌;系统发育地位研究进一步确定2株野生大型真菌为柱状田头菇。结合形态学和分子生物学的方法能准确对柱状田头菇进行分类和鉴定,能显著提高大型野生真菌鉴定的准确率。...

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Published in西南农业学报 Vol. 28; no. 2; pp. 718 - 722
Main Author 李小林 黄羽佳 郑林用 甘炳成 周洁 黄忠乾
Format Journal Article
LanguageChinese
Published 四川省农业科学院土壤肥料研究所,四川成都,610066%四川省技术创新促进会,四川成都,610017 2015
Subjects
ITS
大型真菌
茶树菇
野生资源
Wild resources
茶树菇
Agrocybe cylindracea
Macrofungi
ITS
野生资源
大型真菌
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Summary:为了鉴定2株从四川省成都市法国梧桐树干上采集到的耐高温野生大型真菌,为其开发利用与驯化栽培奠定基础。在形态学特征观察的同时提取了子实体基因组总DNA,并对其模板进行ITS序列的扩增、测序及系统发育地位分析,并将形态学特征和系统发育地位结合来鉴定其准确分类。结果表明：从形态学上可初步鉴定2个菌株均为伞菌目（Agaricales）球盖菇科（Strophariaceae）田头菇属（Agrocybe）大型真菌;系统发育地位研究进一步确定2株野生大型真菌为柱状田头菇。结合形态学和分子生物学的方法能准确对柱状田头菇进行分类和鉴定,能显著提高大型野生真菌鉴定的准确率。
Bibliography:51-1213/S
Wild resources Agrocybe cylindracea ITS Macrofungi
LI Xiao-lin , HUANG Yu-jia , ZHENG Lin-yong , GAN Bing-cheng , ZHOU Jie , HUANG Zhong-qian （1. Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Sichuan Chengdu 610066, China; 2. Technology Innovation Foundation of Sichuan, Sichnan Chengdu 610017, China）
To identify and lay the foundation for the exploitation,domestication,and cultivation of two high temperature resistance wild mushrooms collected from the trunk of Platanus orientalis in Chengdu,Sichuan province. Genomic DNA was extracted from fruiting bodies then used as template for ITS gene PCR amplification while morphology of fruiting bodies was observed. The PCR products of ITS gene were sequenced and the sequences were analyzed using phylogenetic method. The exact classification of fungi was identified by morphological characteristics and phyloenetic position. The results of morphological characteristics indicated that the fungi belonged to order Agaricales,family Strophar
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2015.02.049