三七主要病害生防真菌F2的抑菌作用测定及其鉴定

从三七根际土壤里初步分离筛选到一株对三七多个病原菌具有抑菌作用的真菌F2,研究测试其对三七黑斑病菌（Alternaria panax）、炭疽病菌（Colletotrichum gloeosporioides）和根腐病菌（Fusarium solani）的抑菌率及其对各病原菌菌丝形态的影响。结果表明,真菌F2对三七黑斑病菌、炭疽病菌和根腐病菌的抑菌率较高,分别为88.67%、91.00%和75.76%。显微观察发现,真菌F2可造成三七黑斑病菌和炭疽病菌菌丝膨大、畸形、断裂,造成三七根腐病菌菌丝断裂。结合形态学和分子生物学特征将生防真菌F2鉴定为棘孢木霉（Trichoderma asperellu...

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Published in西南农业学报 Vol. 28; no. 5; pp. 2112 - 2115
Main Author 叶云峰 付岗 胡凤云 蒋妮 刘丽辉 白隆华
Format Journal Article
LanguageChinese
Published 广西壮族自治区药用植物园,广西南宁,530023%广西农业科学院微生物研究所,广西南宁,530007 2015
Subjects
三七病害
抑菌作用
生防真菌
鉴定
生防真菌
抑菌作用
三七病害
鉴定
Disease of Panax notoginseng
Biocontrol fungus
Inhibition effect
Identification
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Summary:从三七根际土壤里初步分离筛选到一株对三七多个病原菌具有抑菌作用的真菌F2,研究测试其对三七黑斑病菌（Alternaria panax）、炭疽病菌（Colletotrichum gloeosporioides）和根腐病菌（Fusarium solani）的抑菌率及其对各病原菌菌丝形态的影响。结果表明,真菌F2对三七黑斑病菌、炭疽病菌和根腐病菌的抑菌率较高,分别为88.67%、91.00%和75.76%。显微观察发现,真菌F2可造成三七黑斑病菌和炭疽病菌菌丝膨大、畸形、断裂,造成三七根腐病菌菌丝断裂。结合形态学和分子生物学特征将生防真菌F2鉴定为棘孢木霉（Trichoderma asperellum）。
Bibliography:Disease of Panax notoginseng; Biocontrol fungus; Inhibition effect; Identification
YE Yun-feng;FU Gang;HU Feng-yun;JIANG Ni;LIU Li-hui;BAI Long-hua （ 1. Guangxi Botanical Garden of Medicinal Plants, Guangxi Nanning 530023, China; 2. Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi Nanning 530007, China）
51-1213/S
The inhibition rates of biocontrol fungus F2 on main pathogenic fungi of Panax notoginseng including Alternaria panax,Colletotrichum gloeosporioides and Fusarium solani were tested in this study. The effects of strain F2 on the mycelia growth of the 3 pathogenic fungi were also observed under the microscope. The results showed that strain F2 could inhibit A. panax,C. gloeosporioides and F. solani obviously with the high inhibition rates of 88. 67 %,91. 00 % and 75. 76 % respectively. When observed under the microscope,mycelia growths of the 3 pathogenic fungi were affected obviously by strain F2. The mycelia of A. panax and C. gloeosporioides showed swollen,abnormal and
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2015.05.049