质子辐照对金花葵生长发育的影响及其基因组变异的RAPD分析

对金花葵（Abelmoschus manihot）种子进行不同剂量的质子辐照,并经过种植观察和RAPD分子标记分析,探究辐照对其生长发育的影响。结果显示,辐照剂量为200、500、1 000 c的金花葵营养生长和生殖生长状况与对照差异显著,但辐照100 c的金花葵变化不明显。其中,辐照剂量为200、500、1 000 c的金花葵植株明显较对照高,茎秆直径也大于对照,但果实的长、宽均有所减小;每果结籽数有一定增多,但种子的千粒重有一定下降,其中以辐照500、1 000 c下降较为明显。RAPD分析显示：各辐照处理谱带的分子量集中在200-2 000 bp之间;各辐照处理的DNA基因组均发生了一定...

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Bibliographic Details
Published in广东农业科学 Vol. 42; no. 14; pp. 41 - 47
Main Author 李佳 周瑜 张启明 吉建斌 李清清 周云龙 苏颖 梁前进
Format Journal Article
LanguageChinese
Published 北京师范大学射线束技术与材料改性教育部重点开放实验室,北京100875%北京师范大学核科学与技术学院,北京,100875 2015
北京师范大学生命科学学院,北京,100875%廊坊市农林科学院花卉研究所,河北廊坊,065000%北京师范大学生命科学学院,北京100875
Subjects
RAPD
生长发育
质子辐照
金花葵
生长发育
质子辐照
RAPD
金花葵
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Summary:对金花葵（Abelmoschus manihot）种子进行不同剂量的质子辐照,并经过种植观察和RAPD分子标记分析,探究辐照对其生长发育的影响。结果显示,辐照剂量为200、500、1 000 c的金花葵营养生长和生殖生长状况与对照差异显著,但辐照100 c的金花葵变化不明显。其中,辐照剂量为200、500、1 000 c的金花葵植株明显较对照高,茎秆直径也大于对照,但果实的长、宽均有所减小;每果结籽数有一定增多,但种子的千粒重有一定下降,其中以辐照500、1 000 c下降较为明显。RAPD分析显示：各辐照处理谱带的分子量集中在200-2 000 bp之间;各辐照处理的DNA基因组均发生了一定变化,出现了条带增多或缺失,以及条带深浅的变化。辐照100、200、500、1 000 c处理电泳条带与对照间的Nei＇s遗传距离分别为0.1126、0.2444、0.2081和0.2000。可见,质子辐照能够引起金花葵的基因组发生变化,并表现出植株增高、茎秆变粗、结籽增多、果实变狭长、种子千粒重降低等生物学效应,辐照剂量在200~1000 c时这种诱变效应更明显。
Bibliography:In this study,the seeds of Abelmoschus manihot were irradiated with different dose of proton irradiation, then the seeds were planted, observed and analysed by RAPD （Randomly Amplified Polymorphic DNA） molecular marker assay method to explore the irradiation effects on their growth and development. The results showed that the vegetative growth and reproductive growth of A. manihot which were irradiated by the dose of 200 c （1 c = 4.6×10^8 protons/cm^2）, 500 c and 1 000 c was obviously different from that of control group. The plants were significantly higher and their stem diameters were greater than those of control group. The length and width of fruits reduced and the seed number of every fruit increased when the seed kernels fell. But the change with the dose of 100 c was not obvious. The RAPD analysis showed that the molecular weight of irradiation groups focused on the band between 200 bp to 2 000 bp. The genomic DNA of every irradiation group changed to a certain degree, with electrophoretic bands incre
ISSN:1004-874X