Accumulation of (E)-4-Hydroxy-2-nonenal and n-Hexanal, Degradation Products of Lipid Peroxides, in Mouse Lung and Liver

• Published in: Biological & pharmaceutical bulletin Vol. 16; no. 1; pp. 84 - 86
• Main Authors: SANO, Mitsuaki, HAGIWARA, Makoto, FUJITA, Masahiro, YOSHINO, Kyoji, TOMITA, Isao
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 01.01.1993; Maruzen; Japan Science and Technology Agency
• Subjects: 4-hydroxy nonenal; Aldehydes - administration & dosage; Aldehydes - blood; Aldehydes - metabolism; Aldehydes - toxicity; Animals; Biological and medical sciences; glutathione; Glutathione - metabolism; glutathione peroxidase; Glutathione Peroxidase - metabolism; glutathione reductase; Glutathione Reductase - metabolism; Injections, Intravenous; Lipid Peroxidation; Liver - drug effects; Liver - metabolism; Lung - drug effects; Lung - metabolism; Male; Medical sciences; Mice; mouse lung; n-hexanal; Pneumology; Respiratory system : syndromes and miscellaneous diseases; Thiobarbituric Acid Reactive Substances - analysis; Glutathione peroxidase; Respiratory disease; Enzyme; Rodentia; Glutathione reductase (NAD(P)H); Hepatic disease; Lipids; Vertebrata; Mammalia; Mouse; Animal; Digestive diseases; Peroxides
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.16.84

Effects of lipid peroxide breakdown products, (E)-4-hydroxy-2-nonenal (4-HN) and n-hexanal, on mouse lung lesion were examined. When 4-HN was injected i.v., the plasuma level of 4-HN increased just after the injection and then decreased immediately. The amounts of 4-HN increased in the liver and lung were ca. 0.085 and 0.43% to the dose administered, respectively, 5min after the injection. Reduced glutathione (GSH) content and both GSH peroxidase (GSH-Px) and GSH reductase (GSSGR) activities in the lung were decreased significantly by 4-HN treatment. On the other hand, in the case of i.v. injection of n-hexanal into mice, the amount of n-hexanal detected in the lung was 5.0% to that of 4-HN, and no effect on the activities of GSH-Px and GSSGR and the content of GSH was observed.These results suggest that 4-HN generated from lipid peroxides would be transferred into the lung and cause the lung lesion through the inhibition of GSH-dependent antioxidative defense systems.