Discovery and demonstration of the temperature stress response functions of Dermatophagoides farinae proteins 1 and 2

• Published in: BMC genomics Vol. 25; no. 1; pp. 811 - 16
• Main Authors: Zhang, Wanyu, Niu, Dongling, Zhao, Yae, Hu, Li, Guan, Chenglin, Chai, Rong
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 28.08.2024; BioMed Central; BMC
• Subjects: Allergens; Allergic diseases; Allergic reaction; Allergies; Allergy; Animals; Annotations; Arthropod Proteins - genetics; Arthropod Proteins - metabolism; Cold; Datasets; Dermatophagoides farinae; DFP1 and 2; Electrophoresis; Environmental aspects; Expression level; Flour; Gene expression; Genes; Genetic aspects; Genomes; Heat; House-dust mite; Mass spectrometry; Mass spectroscopy; Molecular biology; Phylogenetics; Physiological aspects; Polymerase chain reaction; Proteins; Response functions; RNA; Scientific imaging; Sequences; Software; Stress (Physiology); Stress, Physiological; Survival; Survival rate of mite; Temperature; Temperature stress; Transcriptomes; China; United States > US; DFP1 and 2; Survival rate of mite; Temperature stress; Dermatophagoides farinae; Expression level
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-024-10715-2

Dermatophagoides farinae proteins (DFPs) are abundantly expressed in D. farinae; however, their functions remain unknown. Our previous transcriptome sequencing analyses revealed that the basal expression of DFP1 and DFP2 in D. farinae was high and, more importantly, upregulated under temperature stress. Therefore, DFPs were speculated to exert a temperature stress response function. Real-time quantitative polymerase chain reaction detection revealed that both DFP1 and DFP2 were significantly upregulated under temperature stress. Particularly, DFP1 was upregulated under cold stress. Electrophoresis of D. farinae total proteins revealed an increased abundance of DFP1 and DFP2 (40-55 kDa bands) under temperature stress, which was corroborated by the mass spectrometry results. After silencing DFP1 and DFP2 further, temperature stress led to decreases in gene expression and survival rates. Moreover, DFP1 was identified as the upstream regulator of DFP2. This study highlights the temperature stress response functions of DFP1 and DFP2 at the mRNA and protein levels. These results provide important insights for applying DFP1 and DFP2 as potential target genes for the molecular prevention and control of D. farinae to prevent allergic diseases. The newly established methods provide methodological guidance for the study of genes with unknown functions in mites.