Entomopathogenic fungus treatment changes the gut bacterial diversity of Rhipicephalus microplus ticks

• Published in: Parasites & vectors Vol. 16; no. 1; p. 185
• Main Authors: Mesquita, Emily, da Costa, Diogo Paes, Meirelles, Laura Nóbrega, Camargo, Mariana Guedes, Corrêa, Thaís Almeida, Bittencourt, Vânia Rita Elias Pinheiro, da Silva Coelho, Irene, Santos, Huarrisson Azevedo, Humber, Richard Alan, Golo, Patrícia Silva
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 06.06.2023; BioMed Central; BMC
• Subjects: 16S rRNA; Acaricides; Animal health; Animals; Anti-Bacterial Agents - pharmacology; Antibiotics; Antifungal agents; Arachnids; Bacteria; Biological control; Blood; Bovine diseases; Cattle; Cockroaches; Control; Deoxyribonucleic acid; Disease transmission; DNA; Economic impact; Ectoparasites; Entomopathogenic fungi; Female; Females; Fungi; Fungi, Pathogenic; Gastrointestinal Microbiome; Humans; Insect pests; Intestinal microflora; Metarhizium; Metarhizium anisopliae; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Parasites; Parasitic diseases; Pathogens; Pest Control, Biological; Pests; Physiological aspects; Rhipicephalus - microbiology; Rhipicephalus microplus; RNA; RNA, Ribosomal, 16S - genetics; rRNA 16S; Species diversity; Survival; Testing; Tetracycline; Tetracyclines; Ticks; Variable region; Vector-borne diseases; Brazil; United States > US; Rio de Janeiro Brazil; Vector-borne diseases; Bovine diseases; 16S rRNA; Metarhizium anisopliae; Biological control; Tetracycline
• ISSN: 1756-3305; 1756-3305
• DOI: 10.1186/s13071-023-05790-5

Ticks are obligate bloodsucking parasites responsible for significant economic losses and concerns with human and animal health, mainly due to the transmission of pathogens. Entomopathogenic fungi have been intensively studied as an alternative strategy for tick control that can be used in combination with synthetic acaricides in the integrated management of ticks. Here, we investigated how the gut bacterial community of Rhipicephalus microplus is shaped after Metarhizium anisopliae treatment and how the tick susceptibility to the fungus is affected after disrupting gut bacterial microbiota. Partially engorged tick females were artificially fed with pure bovine blood or blood plus tetracycline. Two other groups received the same diet and were topically treated with M. anisopliae. The guts were dissected, and the genomic DNA was extracted 3 days after the treatment; the V3-V4 variable region of the bacterial 16S rRNA gene was amplified. The gut of ticks that received no antibiotic but were treated with M. anisopliae exhibited lower bacterial diversity and a higher occurrence of Coxiella species. The Simpson diversity index and Pielou equability coefficient were higher in the gut bacterial community when R. microplus were fed with tetracycline and fungus-treated. Ticks from fungus-treated groups (with or without tetracycline) exhibited lower survival than untreated females. Previous feeding of ticks with the antibiotic did not change their susceptibility to the fungus. Ehrlichia spp. were not detected in the gueated groups. These findings suggest that myco-acaricidal action would not be impacted if the calf hosting these ticks is under antibiotic therapy. Moreover, the hypothesis that entomopathogenic fungi can affect the bacterial community in the gut of R. microplus engorged females is endorsed by the fact that ticks exposed to M. anisopliae exhibited a dramatic reduction in bacterial diversity. This is the first report of an entomopathogenic fungus affecting the tick gut microbiota.