Transfer of plasmid and chromosomal glycopeptide resistance determinants occurs more readily in the digestive tract of mice than in vitro and exconjugants can persist stably in vivo in the absence of glycopeptide selection

• Published in: Journal of antimicrobial chemotherapy Vol. 59; no. 3; pp. 478 - 486
• Main Authors: Dahl, Kristin Hegstad, Mater, Denis D. G., Flores, María José, Johnsen, Pål Jarle, Midtvedt, Tore, Corthier, Gerard, Sundsfjord, Arnfinn
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.03.2007; Oxford Publishing Limited (England); Oxford University Press (OUP)
• Subjects: Animals; Antibiotics; Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacteria; Bacterial Proteins - genetics; Biological and medical sciences; Carbon-Oxygen Ligases - genetics; Conjugation, Genetic; Drug resistance; Enterococcus; Enterococcus faecium; Gastrointestinal Tract - microbiology; Genetics; Humans; Life Sciences; Male; Medical sciences; Medicin och hälsovetenskap; Mice; Peptides; Pharmacology. Drug treatments; Plasmids; Rodents; vanA; vanB; vancomycin; Vancomycin Resistance - genetics; vancomycin; Peptides; Selection; vanB; vanA; Vancomycin; Glycopeptide; Bacteria; Micrococcales; Genetics; Digestive tract; Enterococcus; Rodentia; Chromosome; In vitro; Plasmid; Resistance; Streptococcaceae; In vivo; Vertebrata; Antibiotic; Mammalia; Mouse; Polypeptide; Animal; Transfer; Antibacterial agent
• ISSN: 0305-7453; 1460-2091
• DOI: 10.1093/jac/dkl530

Objectives and methods The transferability of vanA and vanB glycopeptide resistance determinants with a defined plasmid (n = 9) or chromosomal (n = 4) location between Enterococcus faecium strains of human and animal origins was compared using filter mating (in vitro) and germ-free mice (in vivo) as experimental models. Moreover, the stability of exconjugants in vivo in the absence of antibiotic selection was examined. Results Higher transfer rates were observed in vivo for four of six vanA and five of six vanB donor strains. For plasmid-encoded resistance, several log higher transfer frequencies were observed in vivo for some strains. Moreover, the in vivo model supported transfer of plasmid-encoded vanB (1 × 10−7 exconjugants/donor) when repeated in vitro experiments were negative (estimated < 1 × 10−9 exconjugants/donor). Readily detectable transfer of plasmid-located vanA and vanB as well as large chromosomal (>200 kb) vanB elements was observed after 24 h. The number of plasmid-mediated vanA exconjugants generally decreased markedly after 3 days. However, exconjugants containing a plasmid harbouring the vanA transposon Tn1546 linked to the post-segregational killing system ω-ε-ζ persisted stably in vivo in the absence of glycopeptides for more than 20 days. Conclusions The overall results support the notion that the in vitro model underestimates the transfer potential. Rapid transfer of vanA plasmids from poultry- and pig-derived strains to human faecal E. faecium shows that even transiently colonizing strains may provide a significant reservoir for transfer of resistance genes to the permanent commensal flora. Newly acquired resistance genes may be stabilized and persist in new populations in the absence of antibiotic selection.