Low representation of Fc receptor-like 1-5 molecules in leukemic cells from Iranian patients with acute lymphoblastic leukemia

• Published in: CANCER IMMUNOLOGY IMMUNOTHERAPY Vol. 58; no. 6; pp. 989 - 996
• Main Authors: Kazemi, Tohid, Asgarian-Omran, Hossein, Memarian, Ali, Shabani, Mahdi, Sharifian, Ramazan A, Vossough, Parvaneh, Ansaripour, Bita, Rabbani, Hodjatallah, Shokri, Fazel
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.06.2009; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Adult; Antineoplastic agents; Biological and medical sciences; Cancer; Cancer Research; Child; Flow Cytometry; Fluorescent Antibody Technique, Indirect; Genes; Hematologic and hematopoietic diseases; Hematology; Hospitals; Humans; Immunology; Immunophenotyping; Immunotherapy; Iran - epidemiology; Leukemia; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphoma; Medical sciences; Medicin och hälsovetenskap; Medicine; Medicine & Public Health; Oncology; Pharmacology. Drug treatments; Precursor Cell Lymphoblastic Leukemia-Lymphoma - epidemiology; Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics; Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism; Protein expression; Proteins; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; Receptors, Fc - genetics; Receptors, Fc - metabolism; Receptors, Immunologic - genetics; Receptors, Immunologic - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Short Communication; Iran; Asia; United States > US; Flow cytometry; Acute lymphoblastic leukemia; Immunophenotype; RT-PCR; FCRL; RNA-directed DNA polymerase; Immunoglobulin receptor; Molecules; Lymphoproliferative syndrome; Immunotherapy; Acute lymphocytic leukemia; Cell; Reverse transcription polymerase chain reaction; Human; Enzyme; Transferases; Patient; Malignant hemopathy; Representation; Gene expression; In vitro; FCRL; Acute lymphoblastic leukemia; Nucleotidyltransferases; Treatment; FCRL receptor; Molecular biology; Cancer
• ISSN: 0340-7004; 1432-0851; 1432-0851
• DOI: 10.1007/s00262-008-0589-z

Recent studies have demonstrated expression of Fc receptor-like (FCRL) molecules, a newly identified family with preferential B-cell lineage expression, in some chronic B-cell leukemias with possible implication for classification and/or targeted immunotherapy. In this study, the expression pattern of FCRL1-5 genes was studied in 73 Iranian ALL patients and 35 normal subjects using semi-quantitative RT-PCR method. FCRL protein expression was also investigated by flow cytometry. Our results indicate significant down-regulation of all FCRL genes in ALL compared to normal subjects. Although, FCRL mRNA expression was almost exclusively confined to normal isolated B-cells compared to T-cells, but these genes were similarly expressed in B-ALL, T-ALL and different B-ALL immunophenotypic subtypes. Surface protein expression of FCRL1, 2, 4, and 5 molecules in 10 ALL and 5 normal samples confirmed the PCR results. Expression profile of FCRL molecules in different subtypes of ALL argues against their potential implication as suitable targets for classification and/or immunotherapy of ALL.