A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration

Abstract Management of intestinal failure remains a clinical challenge and total parenteral nutrition, intestinal elongation and/or transplantation are partial solutions. In this study, using a detergent-enzymatic treatment (DET), we optimize in rats a new protocol that creates a natural intestinal...

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Published inBiomaterials Vol. 33; no. 12; pp. 3401 - 3410
Main Authors Totonelli, Giorgia, Maghsoudlou, Panagiotis, Garriboli, Massimo, Riegler, Johannes, Orlando, Giuseppe, Burns, Alan J, Sebire, Neil J, Smith, Virpi V, Fishman, Jonathan M, Ghionzoli, Marco, Turmaine, Mark, Birchall, Martin A, Atala, Anthony, Soker, Shay, Lythgoe, Mark F, Seifalian, Alexander, Pierro, Agostino, Eaton, Simon, De Coppi, Paolo
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Ltd 01.04.2012
Elsevier Science
Subjects
Advanced Basic Science
Animals
Biomechanical Phenomena
Cell Survival
Decellularization
Dentistry
Extracellular Matrix - chemistry
Gut transplantation
Intestinal failure
Intestine, Small - chemistry
Intestine, Small - cytology
Intestine, Small - transplantation
Intestine, Small - ultrastructure
Intestines - physiology
Natural acellular matrix
Rats
Rats, Sprague-Dawley
Regeneration
Regenerative medicine
Regenerative Medicine - methods
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds - chemistry
Natural acellular matrix
Tissue engineering
Regenerative medicine
Intestinal failure
Decellularization
Gut transplantation
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Summary:Abstract Management of intestinal failure remains a clinical challenge and total parenteral nutrition, intestinal elongation and/or transplantation are partial solutions. In this study, using a detergent-enzymatic treatment (DET), we optimize in rats a new protocol that creates a natural intestinal scaffold, as a base for developing functional intestinal tissue. After 1 cycle of DET, histological examination and SEM and TEM analyses showed removal of cellular elements with preservation of the native architecture and connective tissue components. Maintenance of biomechanical, adhesion and angiogenic properties were also demonstrated strengthen the idea that matrices obtained using DET may represent a valid support for intestinal regeneration.
Bibliography:ObjectType-Article-1
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ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2012.01.012