cDNA cloning and primary structure of a white-face hornet venom allergen, antigen 5

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 85; no. 3; pp. 895 - 899
• Main Authors: Fang, K.S.Y, Vitale, M, Fehlner, P, King, T.P
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.02.1988; National Acad Sciences
• Subjects: ADN; ALERGENOS; ALLERGENE; ALLERGENS; Amino Acid Sequence; Amino acids; Animals; Antibodies; ANTIGENE; ANTIGENOS; ANTIGENS; Antigens - biosynthesis; Antigens - genetics; Antigens - immunology; Bacteriophages; Base Sequence; Bee Venoms - genetics; Biological and medical sciences; Biotechnology; CHEMISTRY; CHIMIE; Complementary DNA; DNA; DNA - genetics; DOLICHOVESPULA; Dolichovespula maculata; dolichovespula muculata; Fundamental and applied biological sciences. Psychology; Gels; Genetic engineering; Genetic technics; HYMENOPTERA; Hymenoptera - genetics; IMMUNOLOGIE; IMMUNOLOGY; INMUNOLOGIA; Methods. Procedures. Technologies; Molecular cloning; Molecular Sequence Data; Neurotoxins - genetics; Nicotiana - genetics; Plant antigens; Plant Proteins - genetics; Plants, Toxic; Plasmids; PROTEINAS; PROTEINE; PROTEINS; QUIMICA; Recombinant Proteins - biosynthesis; Recombinant Proteins - immunology; Sequence Homology, Nucleic Acid; VENENO DE SERPIENTES; VENIN; VENOMS; Vespidae; Wasp Venoms - biosynthesis; Wasp Venoms - genetics; Wasp Venoms - immunology; Wasps - genetics; Insecta; Primary structure; Antigen; Dolichovespula maculata; Complementary DNA; Arthropoda; Animal; Venom; Vespidae; Hymenoptera; Molecular cloning; Invertebrata; Aculeata; Allergen

A major allergen of white-face hornet (Dolichovespula maculata) venom is antigen 5 (also designated Dol m V). We have determined the primary structures of two forms of this protein by cDNA and protein sequencings. These two forms with 204 and 205 amino acid residues differ in 23% of their sequences...