cDNA cloning and primary structure of a white-face hornet venom allergen, antigen 5

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 85; no. 3; pp. 895 - 899
• Main Authors: Fang, K.S.Y, Vitale, M, Fehlner, P, King, T.P
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.02.1988; National Acad Sciences
• Subjects: ADN; ALERGENOS; ALLERGENE; ALLERGENS; Amino Acid Sequence; Amino acids; Animals; Antibodies; ANTIGENE; ANTIGENOS; ANTIGENS; Antigens - biosynthesis; Antigens - genetics; Antigens - immunology; Bacteriophages; Base Sequence; Bee Venoms - genetics; Biological and medical sciences; Biotechnology; CHEMISTRY; CHIMIE; Complementary DNA; DNA; DNA - genetics; DOLICHOVESPULA; Dolichovespula maculata; dolichovespula muculata; Fundamental and applied biological sciences. Psychology; Gels; Genetic engineering; Genetic technics; HYMENOPTERA; Hymenoptera - genetics; IMMUNOLOGIE; IMMUNOLOGY; INMUNOLOGIA; Methods. Procedures. Technologies; Molecular cloning; Molecular Sequence Data; Neurotoxins - genetics; Nicotiana - genetics; Plant antigens; Plant Proteins - genetics; Plants, Toxic; Plasmids; PROTEINAS; PROTEINE; PROTEINS; QUIMICA; Recombinant Proteins - biosynthesis; Recombinant Proteins - immunology; Sequence Homology, Nucleic Acid; VENENO DE SERPIENTES; VENIN; VENOMS; Vespidae; Wasp Venoms - biosynthesis; Wasp Venoms - genetics; Wasp Venoms - immunology; Wasps - genetics; Insecta; Primary structure; Antigen; Dolichovespula maculata; Complementary DNA; Arthropoda; Animal; Venom; Vespidae; Hymenoptera; Molecular cloning; Invertebrata; Aculeata; Allergen
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.85.3.895

A major allergen of white-face hornet (Dolichovespula maculata) venom is antigen 5 (also designated Dol m V). We have determined the primary structures of two forms of this protein by cDNA and protein sequencings. These two forms with 204 and 205 amino acid residues differ in 23% of their sequences but they are antigenically similar. Both forms have sequence similarity with a pathogenesis-related protein of tobacco leaf. In a 130-residue overlap of these proteins, 35-39 residues were identical. Hornet antigen 5 cDNAs were isolated from an expression library in λ gt11 phage using antibody probes. Several of the cDNAs were not full length, but the fusion fragments expressed were immunoreactive. These results suggest that antigenic determinants of the sequential type are distributed throughout the entire molecule of antigen 5. After subcloning, antigen 5 was also expressed in pKK233-2 plasmid.