Superior protection elicited by live-attenuated vaccines in the murine model of paratuberculosis

• Published in: Vaccine Vol. 33; no. 51; pp. 7262 - 7270
• Main Authors: Ghosh, Pallab, Shippy, Daniel C, Talaat, Adel M
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 16.12.2015; Elsevier Limited
• Subjects: Adjuvant; Adjuvants, Immunologic - administration & dosage; Allergy and Immunology; Animal Structures - pathology; Animals; Bacterial Load; Bacterial Vaccines - administration & dosage; Bacterial Vaccines - immunology; Cattle; Dairy industry; Disease Models, Animal; Economic impact; Female; Gene Knockout Techniques; Immunogenicity; Interferon-gamma - secretion; Johne's disease; Leukocytes, Mononuclear - immunology; Live attenuated vaccine; Mice, Inbred C57BL; Milk; Mycobacterium avium; Mycobacterium avium subsp. paratuberculosis; Mycobacterium avium subsp. paratuberculosis - genetics; Mycobacterium avium subsp. paratuberculosis - immunology; Paratuberculosis - immunology; Paratuberculosis - pathology; Paratuberculosis - prevention & control; Pasteurization; Quillaja Saponins - administration & dosage; Ruminantia; Sigma Factor - deficiency; Sigma factor sigH; Vaccines; Vaccines, Attenuated - administration & dosage; Vaccines, Attenuated - immunology; Virulence; Virulence Factors - deficiency; Sigma factor sigH; Adjuvant; Mycobacterium avium subsp. paratuberculosis; Live attenuated vaccine; Johne's disease
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2015.10.116

Highlights • Novel vaccines are needed to control Johne's disease distressing the dairy industry. • LAVs provide protection against M. paratuberculosis by stimulating host immunity. • Δ sigH LAV was safe and highly immunogenic in murine model of paratuberculosis. • Δ sigH LAV performed better than the available licensed vaccine for Johne's disease. • The used approach could be useful against other intracellular pathogens.