Bone Healing Improvements Using Hyaluronic Acid and Hydroxyapatite/Beta-Tricalcium Phosphate in Combination: An Animal Study

• Published in: BioMed research international Vol. 2016; no. 2016; pp. 1 - 8
• Main Authors: Huang, Haw-Ming, Lin, Che-Tong, Tsai, Hsin-Yuan, Huang, Yu-Chih, Feng, Sheng-Wei, Lo, Yi-June, Chang, Yen-Lan, Fan, Kan-Hsin
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2016; John Wiley & Sons, Inc
• Subjects: Acids; Animals; Bone and Bones - diagnostic imaging; Bone and Bones - pathology; Bone and Bones - surgery; Bone Diseases - therapy; Bone Regeneration; Bone Substitutes - chemistry; Bones; Calcium phosphate; Cell adhesion & migration; Composite materials; Density; Dentistry; Disease Models, Animal; Efficiency; Hospitals; Hyaluronic acid; Hydroxyapatite; Hydroxyapatites - chemistry; Osteogenesis; Particle size; Physical properties; Powders; Rabbits; Sinuses; Skull - pathology; Studies; Tissue engineering; Transmission Control Protocol/Internet Protocol (Computer network protocol); Transplants & implants; Viscoelasticity; X-Ray Microtomography; Taiwan
• ISSN: 2314-6133; 2314-6141; 2314-6141
• DOI: 10.1155/2016/8301624

The purpose of this study was to investigate whether the use of HLA as an aqueous binder of hydroxyapatite/beta-tricalcium phosphate (HA-βTCP) particles can reduce the amount of bone graft needed and increase ease of handling in clinical situations. In this study, HA/βTCP was loaded in commercially available crosslinking HLA to form a novel HLA/HA-βTCP composite. Six New Zealand White rabbits (3.0–3.6 kg) were used as test subjects. Four 6 mm defects were prepared in the parietal bone. The defects were filled with the HLA/HA-βTCP composite as well as HA-βTCP particle alone. New bone formation was analyzed by micro-CT and histomorphometry. Our results indicated that even when the HA-βTCP particle numbers were reduced, the regenerative effect on bone remained when the HLA existed. The bone volume density (BV/TV ratio) of HLA/HA-βTCP samples was 1.7 times larger than that of the control sample at week 2. The new bone increasing ratio (NBIR) of HLA/HA-βTCP samples was 1.78 times higher than the control group at week 2. In conclusion, HA-βTCP powder with HLA contributed to bone healing in rabbit calvarial bone defects. The addition of HLA to bone grafts not only promoted osteoconduction but also improved handling characteristics in clinical situations.