An ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5 gene mutation confers light green peel in cucumber

• Published in: Journal of integrative plant biology Vol. 57; no. 11; pp. 936 - 942
• Main Authors: Zhou, Qian, Wang, Shenhao, Hu, Bowen, Chen, Huiming, Zhang, Zhonghua, Huang, Sanwen
• Format: Journal Article Web Resource
• Language: English
• Published: China (Republic : 1949- ) Blackwell Pub 01.11.2015; Blackwell Publishing Ltd; Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture,Sino-Dutch Joint Laboratory of Horticultural Genomics,Beijing 100081,China; Agricultural Genomic Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China%Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture,Sino-Dutch Joint Laboratory of Horticultural Genomics,Beijing 100081,China%Hunan Vegetable Research Institute,Hunan Academy of Agricultural Science,Changsha 410125,China; Wiley
• Subjects: Agriculture & agronomie; Agriculture & agronomy; alleles; Amino Acid Sequence; amino acids; Arabidopsis; ARC5; breeding; chloroplasts; Chloroplasts - genetics; color; cucumbers; Cucumis sativus; Cucumis sativus - genetics; Cucumis Sativus L; EMS Mutant; ethyl methane sulfonate mutant; ethyl methanesulfonate; exocarp; fruit peel color; Fruit Skin Color; genetic techniques and protocols; green light; guanosinetriphosphatase; Life sciences; Molecular Sequence Data; mutants; phenotype; Pigmentation - genetics; recessive genes; RNA Interference; Sciences du vivant; single nucleotide polymorphism; Whole Genome Re-sequencing; 单核苷酸多态性; 叶绿体发育; 基因突变; 复制; 果皮颜色; 甲基磺酸乙酯; 积累; 黄瓜; ethyl methane sulfonate mutant; Cucumis sativus; fruit peel color; ARC5; whole genome re-sequencing
• ISSN: 1672-9072; 1744-7909; 1744-7909
• DOI: 10.1111/jipb.12355

The peel color of fruit is an important commercial trait in cucumber, but the underlying molecular basis is largely unknown. A mutant showing light green exocarp was discovered from ethyl methane sulfonate （EMS） mutagenized cucumber line 406 with dark green exocarp. Genetic analysis showed the mutant phenotype is conferred by a single recessive gene, here designated as lgp （light green peel）. By re-sequencing of bulked segregants, we identified the candidate gene Csa7Go51430 encoding ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5 （ARCS） that plays a vital role in chloroplast division in Arabidopsis. A single nucleotide polymorphism （SNP） causing amino acid alteration in the conserved GTPase domain of Csa7Go5143o showed co-segregation with the altered phenotype. Furthermore, the transient RNA interference of this gene resulted in reduced number and enlarged size of chloroplasts, which were also observed in the Igp mutant. This evidence supports that the non-synonymous SNP in Csa7G051430 is the causative mutation for the light green peel. This study provides a new allele for cucumber breeding for light green fruits and additional resource for the study of chloroplast development.