Quantification of cellular properties from external fields and resulting induced velocity: Magnetic susceptibility

• Published in: Biotechnology and bioengineering Vol. 64; no. 5; pp. 519 - 526
• Main Authors: Chalmers, Jeffrey J., Haam, Seungjoo, Zhao, Yang, McCloskey, Kara, Moore, Lee, Zborowski, Maciej, Williams, P. Stephen
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 05.09.1999; Wiley
• Subjects: Algorithms; Biological and medical sciences; Biotechnology; Cells; Cells, Cultured; computer imaging; Fundamental and applied biological sciences. Psychology; Humans; Image Processing, Computer-Assisted; Immunology; immunomagnetic labeling; Immunomagnetic Separation; Lymphocytes - cytology; magnetic cell separation; Magnetic field effects; Magnetic separation; Magnetic susceptibility; magnetic susceptibility measurements; Magnetic variables measurement; Methods. Procedures. Technologies; Others; Probes; Tumor Cells, Cultured; Various methods and equipments; Velocity measurement; Human; Particle tracking velocimetry; Separation method; Immunomagnetic method; Cell line; Speed measurement; Mammary gland; Measurement method; Magnetic field; Lymphocyte
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/(SICI)1097-0290(19990905)64:5<519::AID-BIT2>3.0.CO;2-V

An experimental technique is discussed in which the magnetic susceptibility of immunomagnetically labeled cells can be determined on a cell‐by‐cell basis. This technique is based on determining the magnetically induced velocity that an immunomagnetically labeled cell has in a well‐defined magnetic energy gradient. This velocity is determined through the use of video recordings of microscopic images of cells moving in the magnetic energy gradient. These video images are then computer digitized and processed using a computer algorithm, cell tracking velocimetry, which allows larger numbers (>103) of cells to be analyzed. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64:519–526, 1999.