Multiple modes of activation of the stress-responsive MAP kinase pathway in fission yeast
The Schizosaccharomyces pombe wis1+ gene is essential for cell survival under stress conditions. The MAPKK homologue Wis1 is required for activation of the MAPK homologue Spc1, and integrity of the Wis1–Spc1 pathway is required for survival in extreme conditions of heat, osmolarity, oxidation or lim...
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Published in | The EMBO journal Vol. 16; no. 20; pp. 6162 - 6170 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
15.10.1997
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Subjects | |
Online Access | Get full text |
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Summary: | The Schizosaccharomyces pombe wis1+ gene is essential for cell survival under stress conditions. The MAPKK homologue Wis1 is required for activation of the MAPK homologue Spc1, and integrity of the Wis1–Spc1 pathway is required for survival in extreme conditions of heat, osmolarity, oxidation or limited nutrition. We show here that Wis4, a protein kinase of a new MAPKKK class, phosphorylates Wis1 in vitro and activates it in vivo. Win1 is also required for full activation of Wis1, and Win1 rather than Wis4 mediates the osmotic stress signal. Surprisingly, the pathway can still be activated by heat or oxidative stress independently of the phosphorylation of two conserved Wis1 residues. Evidence is presented that the Pyp1 protein tyrosine phosphatase, which dephosphorylates Spc1, is central to this alternative activation mechanism. |
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Bibliography: | ark:/67375/WNG-CKQJ1MCP-Z istex:CB53A05DFEF7A242314D5EE781577D95EE7C986A ArticleID:EMBJ7590590 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0261-4189 1460-2075 1460-2075 |
DOI: | 10.1093/emboj/16.20.6162 |