应用正交试验优化猪细小病毒N株在IBRS-2细胞转瓶培养

利用正交试验对猪细小病毒（PPV）N株在IBRS-2细胞转瓶培养中的培养条件进行优化，为PPVN株弱毒疫苗的大量培养工艺提供参考。设细胞培养液pH值（7．0、7．2和7．4）、接毒时间（0、24和48h）、接毒剂量（0．10％、1．00％和10．oo％）、收毒时间（48、72和96h）等因子和水平。结果表明，PPVN株转瓶培养的最佳培养条件组合为细胞培养液pH值7．2，采用同步接毒，接毒剂量为0．10％，收毒时间为接毒后96h。其中收毒时间是影响PPVN株毒价的最主要因素。...

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Bibliographic Details
Published in西南农业学报 Vol. 30; no. 2; pp. 470 - 473
Main Author 卢冰霞 何颖 赵武 梁家幸 段群棚 蒋冬福 卢敬专 闭炳芬 周英宁 秦毅斌 李斌 苏乾莲 陈忠伟
Format Journal Article
LanguageChinese
Published 广西壮族自治区兽医研究所/广西兽医生物技术重点实验室,广西南宁,530001 2017
Subjects
优化
正交试验
猪细小病毒
转瓶培养
正交试验
猪细小病毒
优化
转瓶培养
Orthogonal experiment
Cells culture using roller bottle
Optimize
Porcine parvovirus
Online AccessGet full text
ISSN1001-4829
DOI10.16213/j.cnki.scjas.2017.2.038

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Summary:利用正交试验对猪细小病毒（PPV）N株在IBRS-2细胞转瓶培养中的培养条件进行优化，为PPVN株弱毒疫苗的大量培养工艺提供参考。设细胞培养液pH值（7．0、7．2和7．4）、接毒时间（0、24和48h）、接毒剂量（0．10％、1．00％和10．oo％）、收毒时间（48、72和96h）等因子和水平。结果表明，PPVN株转瓶培养的最佳培养条件组合为细胞培养液pH值7．2，采用同步接毒，接毒剂量为0．10％，收毒时间为接毒后96h。其中收毒时间是影响PPVN株毒价的最主要因素。
Bibliography:LU Bing-xia, HE Ying, ZHAO Wu, LIANG Jia-xing, DUAN Qun-peng, JIANG Dong-fu, LU Jing-zhuan, BI Bing-fen, ZHOU Ying-ning, QIN Yi-bin, LI Bin, SU Qian-lian, CHEN Zhong-wei （Guangxi Veterinary Research Institute/Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Nanning 530001 ,China）
51-1213/S
Porcine parvovirus; Cells culture using roller bottle; Optimize; Orthogonal experiment
The orthogonal design was used to optimize the culture conditions, of PPV N strain in IBRS-2 cell cultivated in roller bottles to pro- vide reference for the mass culture of PPV N attenuated vaccine. Affected factors were as follows ： the pH value （7.0, 7.2, 7.4）, the time of inoculation （0, 24, 48h）, the dose of toxin （0.10 % , 1.00 %, 10.00 % ） and the time of harvesting （48, 72, 96 h）. Results showed that the best culture conditions of PPV N strain IBRS-2 cells cultivated in roller bottles were as follows： pH =7.2, synchronization inocula- tion, 0.10 % inoculation dose, harvesting the virus solution 96 h after inoculation. Viru
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2017.2.038