广西野生蕉种质资源调查与鉴定
【目的】较全面地收集并鉴定广西野生蕉种质资源的亲缘关系和分类地位,为广西野生蕉资源的保护和利用提供理论依据。【方法】采用染色体制片压片法、形态学分类法和荧光AFLP标记分别对广西境内的14份野生蕉种质进行染色体倍性、形态学分类和亲缘关系的鉴定与研究。【结果】形态学分类鉴定表明,供试的14份野生蕉种质资源染色体倍性均为二倍体,2n=22(x=11)。其中2份鉴定为M.acuminata subsp.malaccensis种,其余12份鉴定为M.itinerans种,种间形态学性状差异较大。基于AFLP标记的鉴定结果表明广西野生蕉资源具有丰富的遗传多样性,与形态学鉴定结果基本一致。【结论】供试的1...
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Published in | 西南农业学报 Vol. 30; no. 6; pp. 1284 - 1293 |
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Main Author | |
Format | Journal Article |
Language | Chinese |
Published |
广西农业科学院园艺研究所,广西南宁,530007
2017
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Subjects | |
Online Access | Get full text |
ISSN | 1001-4829 |
DOI | 10.16213/j.cnki.scjas.2017.6.008 |
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Summary: | 【目的】较全面地收集并鉴定广西野生蕉种质资源的亲缘关系和分类地位,为广西野生蕉资源的保护和利用提供理论依据。【方法】采用染色体制片压片法、形态学分类法和荧光AFLP标记分别对广西境内的14份野生蕉种质进行染色体倍性、形态学分类和亲缘关系的鉴定与研究。【结果】形态学分类鉴定表明,供试的14份野生蕉种质资源染色体倍性均为二倍体,2n=22(x=11)。其中2份鉴定为M.acuminata subsp.malaccensis种,其余12份鉴定为M.itinerans种,种间形态学性状差异较大。基于AFLP标记的鉴定结果表明广西野生蕉资源具有丰富的遗传多样性,与形态学鉴定结果基本一致。【结论】供试的14份野生蕉均属于芭蕉属真蕉组(Eumusa),其中2份归属为小果野蕉亚种,12份归属为阿宽蕉,该种在广西分布较普遍。AFLP鉴定结果显示广西野生蕉种质资源间遗传差异较大,与栽培蕉亲缘关系也较远。 |
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Bibliography: | LONG Xing, QIN Xian-quan, FANG Ren, DENG Biao, AN Zhen-yu, HUANG Wei-xiong, YAO Jin-yan ( Horticultural Research Institute, Guartgxi Academy of Agricultural Sciences, Guangxi Nanning 530007, China) Wild Musa germplasm ; Germplasm resource ; Classification identification ; Morphological marker; AFLP ; Guangxi [ Objective ] The wild Musa germplasms resources were roundly collected in Guangxi province and their genetic relationship and classification status were clarified to provide a theoretical basis for protection and utilization of the wild Musa germplasm. [ Method] Based on chromosome tabletting, morphological taxonomy and AFLP labeled with fluorescence methods, genetic relationship of 14 wild Musa germ- plasms from different regions in Guangxi Province were investigated and studied. [ Result ] The morphological identification showed that the chromosome ploidy of the 14 wild Musa germplasms were diploid, 2n = 22 ( x = 11 ). 2 of them were identified as M. acumirtata subsp malac- censis, and other 12 were iden |
ISSN: | 1001-4829 |
DOI: | 10.16213/j.cnki.scjas.2017.6.008 |