Effect of toothpaste with nano-sized trimetaphosphate on dental caries: In situ study

• Published in: Journal of dentistry Vol. 43; no. 7; pp. 806 - 813
• Main Authors: Danelon, Marcelle, Pessan, Juliano Pelim, Neto, Francisco Nunes Souza, de Camargo, Emerson Rodrigues, Delbem, Alberto Carlos Botazzo
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2015; Elsevier Limited
• Subjects: Adult; Analysis of variance; Animals; Cattle; Clinical trials; Cross-Over Studies; Dental caries; Dental Caries - drug therapy; Dentistry; Enamel; Enamels; Female; Fluorides; Fluorides, Topical - administration & dosage; Hardness - drug effects; Humans; Hydroxyapatite; Lesions; Male; Minerals; Nano-sized; Nanoparticles - administration & dosage; Nanoparticles - chemistry; Nanostructure; Phases; Phosphates; Polyphosphates - administration & dosage; Polyphosphates - pharmacology; Polyphosphates - therapeutic use; Recovery; Sodium; Students; Studies; Tooth Remineralization; Toothpaste; Toothpastes; Toothpastes - chemistry; Toothpastes - therapeutic use; Young Adult; Phosphates; Nano-sized; Toothpastes; Tooth remineralization
• ISSN: 0300-5712; 1879-176X; 1879-176X
• DOI: 10.1016/j.jdent.2015.04.010

This in situ study was to evaluate the remineralizing effect of a fluoride toothpaste supplemented with nano-sized sodium trimetaphosphate (TMP). This blind and cross-over study was performed in 4 phases of 3 days each. Twelve subjects used palatal appliances containing four bovine enamel blocks with artificial caries lesions. Volunteers were randomly assigned into the following treatment groups: Placebo (without F and TMP); 1100ppm F (1100), 1100 supplemented with 3% micrometric TMP (1100 TMP) and with nano-sized TMP (1100 TMPnano). Volunteers were instructed to brush their natural teeth with the palatal appliances in the mouth during 1min (3 times/day), so that blocks were treated with natural slurries of toothpastes. After each phase, the percentage of surface hardness recovery (%SHR), integrated mineral recovery (IMR) and integrated differential mineral area profile (ΔIMR) in enamel lesions were calculated. F in enamel was also determined. Data were analyzed by ANOVA and Student–Newman–Keuls test. Enamel surface became 20% harder when treated with 1100 TMPnano in comparison with 1100 (p<0.001). 1100 TMPnano showed remineralizing capacity (IMR; ΔIMR) 66% higher when compared with 1100 TMP (p<0.001). Enamel F uptake in the 1100 TMPnano group was 2-fold higher when compared to its counterpart without TMP (p<0.001). The addition of 3% TMPnano to a conventional toothpaste was able to promote an additional remineralizing effect of artificial caries lesions. Toothpaste containing 1100ppm F associated with TMPnano showed a potential of higher remineralization to 1100ppm F and 1100ppm F micrometric TMP.