Comparative transcriptome analysis provides insights into the molecular mechanisms of high-frequency hearing differences between the sexes of Odorrana tormota

• Published in: BMC genomics Vol. 23; no. 1; p. 296
• Main Authors: Chen, Zhuo, Liu, Yao, Liang, Rui, Cui, Chong, Zhu, Yanjun, Zhang, Fang, Zhang, Jie, Chen, Xiaohong
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 12.04.2022; BioMed Central; BMC
• Subjects: Acoustics; Adaptation; Amphibians; Analysis; Animal communication; Animals; Annotations; Anura - physiology; Background noise; Behavior; Communication; Dimorphism (Biology); Energy metabolism; Evolution & development; Female; Females; Frogs; Gender aspects; Gender differences; Gene expression; Gene Expression Profiling; Genes; Genetic aspects; Genomes; Genomics; Hearing; Hearing - physiology; High-frequency hearing; Information processing; Ion transport; Male; Males; Mammals; Masking; Methods; Molecular mechanics; Molecular modelling; Neurogenesis; Noise; Observations; Odorrana tormota; Proteins; Ranidae; Sex differences; Sex dimorphism; Sexes; Signal transduction; Transcriptome; Transcriptomes; Ultrasonic imaging; Vertebrates; China; Odorrana tormota; High-frequency hearing; Sex dimorphism; Transcriptome; Adaptation
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-022-08536-2

Acoustic communication is important for the survival and reproduction of anurans and masking background noise is a critical factor for their effective acoustic communication. Males of the concave-eared frog (Odorrana tormota) have evolved an ultrasonic communication capacity to avoid masking by the widespread background noise of local fast-flowing streams, whereas females exhibit no ultrasonic sensitivity. However, the molecular mechanisms underlying the high-frequency hearing differences between the sexes of O. tormota are still poorly understood. In this study, we sequenced the brain transcriptomes of male and female O. tormota, and compared their differential gene expression. A total of 4,605 differentially expressed genes (DEGs) between the sexes of O. tormota were identified and eleven of them were related to auditory based on the annotation and enrichment analysis. Most of these DEGs in males showed a higher expression trend than females in both quantity and expression quantity. The highly expressed genes in males were relatively concentrated in neurogenesis, signal transduction, ion transport and energy metabolism, whereas the up-expressed genes in females were mainly related to the growth and development regulation of specific auditory cells. The transcriptome of male and female O. tormota has been sequenced and de novo assembled, which will provide gene reference for further genomic studies. In addition, this is the first research to reveal the molecular mechanisms of sex differences in ultrasonic hearing between the sexes of O. tormota and will provide new insights into the genetic basis of the auditory adaptation in amphibians during their transition from water to land.