LncRNA TTN-AS1 promotes the progression of oral squamous cell carcinoma via miR-411-3p/NFAT5 axis

• Published in: Cancer cell international Vol. 20; no. 1; p. 415
• Main Authors: Fu, Su-Wei, Zhang, Yan, Li, Shen, Shi, Zhi-Yan, Zhao, Juan, He, Qing-Li
• Format: Journal Article
• Language: English
• Published: England BioMed Central 28.08.2020; BMC
• Subjects: Antisense RNA; Apoptosis; Binding sites; Cell adhesion & migration; Cell culture; Cell growth; Cell migration; Cell proliferation; Connectin; Flow cytometry; Laboratory animals; miR-411-3p; NFAT5; Oral cancer; Oral squamous cell carcinoma; Pathogenesis; Plasmids; Primary Research; Proteins; Public health; Ribonucleic acid; RNA; Squamous cell carcinoma; TTN-AS1; Wound healing; United States > US; China; Japan; NFAT5; miR-411-3p; TTN-AS1; Oral squamous cell carcinoma
• ISSN: 1475-2867; 1475-2867
• DOI: 10.1186/s12935-020-01378-6

Oral squamous cell carcinoma (OSCC) is a common kind of squamous cell carcinoma of the head and neck, which is a threat to public health. Long noncoding RNAs (lncRNAs) are associated with the development of various diseases, including cancers. LncRNA titin antisense RNA 1 (TTN-AS1) is known as a crucial regulatory factor in several cancers. Nevertheless, the specific functions of TTN-AS1 in OSCC remains obscure. The expression of TTN-AS1 in OSCC samples or cells was analyzed through qRT-PCR. Colony formation assay, EdU assay, flow cytometry assay, TUNEL assay and wound healing assay were conducted to estimate the functions of TTN-AS1 in OSCC cells. RIP and luciferase reporter assays were utilized to detect the interaction between TTN-AS1 and miR-411-3p as well as between miR-411-3p and NFAT5. TTN-AS1 expression was stronger in OSCC cells. Knockdown of TTN-AS1 effectively restrained cell proliferation and migration but had inductive role in apoptosis. Moreover, TTN-AS1 could function as the miR-411-3p sponge in OSCC and miR-411-3p exerted the inhibitory functions on OSCC cell growth. In addition, NFAT5 was proven as the target of miR-411-3p. Rescue assay indicated that overexpressing NFAT5 could reverse the inhibitory function of TTN-AS1 depletion on cell growth. lncRNA TTN-AS1 contributed to the progression of OSCC via miR-411-3p/NFAT5 axis.