novel form of nitrogen storage during growth of Paracoccus denitrificans

The uptake of NH$_{4}^{+}$ and subsequent storage of nitrogen by Paracoccus denitrificans during growth was investigated, with capillary gas chromatography as the primary technique for detection and quantitation of associated nitrogenous metabolites. Nitrogen supplied as NH$_{4}^{+}$ was rapidly tak...

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Bibliographic Details
Published inProceedings of the Royal Society of London. Series B, Biological sciences Vol. 227; no. 1249; pp. 429 - 440
Main Authors Dunstan, R.H, Whatley, F.R
Format Journal Article
LanguageEnglish
Published The Royal Society 22.05.1986
Subjects
growth
nitrogen metabolism
Paracoccus denitrificans
soil bacteria
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Summary:The uptake of NH$_{4}^{+}$ and subsequent storage of nitrogen by Paracoccus denitrificans during growth was investigated, with capillary gas chromatography as the primary technique for detection and quantitation of associated nitrogenous metabolites. Nitrogen supplied as NH$_{4}^{+}$ was rapidly taken up by P. denitrificans in the early stages of growth, and the subsequent rapid accumulation of water soluble complexes containing adenine and guanine was observed. When the available NH$_{4}^{+}$ in the culture medium was depleted, the adenine and guanine complexes broke down and enabled the bacterium to continue growth at the initial rate after depletion of the externally available nitrogen. The water-soluble adenine and guanine complexes broke down on acid hydrolysis, yielding free adenine and guanine.
ISSN:0080-4649
0962-8452
2053-9193
1471-2954
DOI:10.1098/rspb.1986.0032