Postprandial effects of a meal low in sulfur amino acids and high in polyunsaturated fatty acids compared to a meal high in sulfur amino acids and saturated fatty acids on stearoyl CoA-desaturase indices and plasma sulfur amino acids: a pilot study

• Published in: BMC research notes Vol. 13; no. 1; p. 379
• Main Authors: Olsen, Thomas, Turner, Cheryl, Øvrebø, Bente, Bastani, Nasser E, Refsum, Helga, Vinknes, Kathrine J
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 10.08.2020; BioMed Central; BMC
• Subjects: Amino acids; Amino Acids, Sulfur; Blood pressure; Body composition; Body fat; Coenzyme A; Confidence intervals; Cysteine; Desaturase; Fatty acid desaturases; Fatty Acids; Fatty Acids, Unsaturated; Homocysteine; Humans; Intervention; Meals; Metabolism; Metabolites; Methionine restriction; Physiological aspects; Pilot Projects; Plasma; Polyunsaturated fatty acids; Research Note; Stearoyl CoA-desaturase; Studies; Sulfur; Sulfur amino acid restriction; Vegan; Vegetarian; Vegetarianism; Norway; United States > US; Cysteine; Stearoyl CoA-desaturase; Sulfur amino acid restriction; Vegetarian; Methionine restriction; Vegan
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-020-05222-y

The sulfur amino acid (SAA) cysteine is positively related, whereas polyunsaturated fatty acids (PUFAs) are inversely related to activity of the lipogenic enzyme stearoyl-CoA desaturase (SCD). High SCD activity promotes obesity in animals, and plasma activity indices positively associates with fat mass in humans. SCD may thus be a target for dietary intervention with SAA restriction and PUFA enrichment with unknown potential benefits for body composition. We randomized ten healthy individuals to a meal restricted in SAAs and enriched with PUFAs (Cys/Met  + PUFA) (n = 5) or a meal enriched in SAA and saturated fatty acids (Cys/Met  + SFA) (n = 5). We measured plasma SCD activity indices (SCD16 and SCD18) and SAAs response hourly from baseline and up to 4 h postprandial. SCD16 was unchanged whereas SCD18 tended to increase in the Cys/Met  + PUFA compared to the Cys/Met  + SFA group (p  = 0.08). Plasma concentrations of total cysteine fractions including free and reduced cysteine decreased in the Cys/Met  + PUFA compared to the Cys/Met  + SFA group (both p  < 0.001). In conclusion, a meal low in SAA but high in PUFAs reduced plasma cysteine fractions but not SCD activity indices. This pilot study can be useful for the design and diet composition of future dietary interventions that targets SCD and SAA. Trial registration ClinicalTrials.gov: NCT02647970, registration date: 6 January 2016.