Isolation of two genes that encode subunits of the yeast transcription factor IIA

• Published in: Science (American Association for the Advancement of Science) Vol. 255; no. 5048; pp. 1127 - 1129
• Main Authors: Ranish, J.A. (Fred Hutchinson Cancer Research Center, Seattle, WA), Lane, W.S, Hahn, S
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 28.02.1992; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Amino Acid Sequence; Amino acids; Biological and medical sciences; Carrier proteins; Cloning, Molecular; Coding; DNA; DNA Mutational Analysis; DNA-Binding Proteins - genetics; Fractions; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genes, Fungal; Genetic aspects; Genetic screening; Genetic transcription; Genetics; LEVADURA; LEVURE; Mobility; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Plasmids; Recombinant Proteins - metabolism; Reticulocytes; RNA; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins; Transcription (Genetics); Transcription Factor TFIIA; Transcription factors; Transcription Factors - genetics; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; Translation; Yeast; Yeast (Food product); Yeasts; Yeast; Gene; Nucleotide sequence; Molecular cloning; Transcription factor; Aminoacid sequence
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1546313

The yeast transcription factor IIA (TFIIA), a component of the basal transcription machinery of RNA polymerase II and implicated in vitro in regulation of basal transcription, is composed of two subunits of 32 and 13.5 kilodaltons. The genes that encode these subunits, termed TOA1 and TOA2, respectively, were cloned. Neither gene shares obvious sequence similarity with the other or with any other previously identified genes. The recombinant factor bound to a TATA binding protein-DNA complex and complemented yeast and mammalian in vitro transcription systems depleted of TFIIA. Both the TOA1 and TOA2 genes are essential for growth of yeast