Dapagliflozin attenuates hypoxia/reoxygenation-caused cardiac dysfunction and oxidative damage through modulation of AMPK

• Published in: Cell & bioscience Vol. 11; no. 1; pp. 44 - 13
• Main Authors: Tsai, Kun-Ling, Hsieh, Pei-Ling, Chou, Wan-Ching, Cheng, Hui-Ching, Huang, Yu-Ting, Chan, Shih-Hung
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 26.02.2021; BioMed Central; BMC
• Subjects: Adenosine; AMPK; Antibodies; Antidiabetics; Apoptosis; Cardiac function; Copy number; Coronary vessels; Dapagliflozin; Dehydrogenases; Dextrose; Echocardiography; Ethylenediaminetetraacetic acid; Glucose; Heart attacks; Hypoxia; Ischemia; Ischemia/reperfusion injury; Kinases; Laboratory animals; Membrane potential; Membranes; Metabolism; Mitochondrial DNA; Myoblasts; Myocardial infarction; Myocardium; NAD(P)H oxidase; Ostomy; Oxidases; Oxidative stress; Penicillin; Phosphorylation; Prevention; Protein kinase C; Proteins; Reperfusion; Sodium-glucose cotransporter; Type 2 diabetes; Canada; United States > US; Dapagliflozin; AMPK; Ischemia/reperfusion injury
• ISSN: 2045-3701; 2045-3701
• DOI: 10.1186/s13578-021-00547-y

Emerging evidence demonstrated dapagliflozin (DAPA), a sodium-glucose cotransporter 2 inhibitor, prevented various cardiovascular events. However, the detailed mechanisms underlying its cardioprotective properties remained largely unknown. In the present study, we sought to investigate the effects of DAPA on the cardiac ischemia/reperfusion (I/R) injury. Results from in vitro experiments showed that DAPA induced the phosphorylation of AMPK, resulting in the downregulation of PKC in the cardiac myoblast H9c2 cells following hypoxia/reoxygenation (H/R) condition. We demonstrated that DAPA treatment diminished the H/R-elicited oxidative stress via the AMPK/ PKC/ NADPH oxidase pathway. In addition, DAPA prevented the H/R-induced abnormality of PGC-1α expression, mitochondrial membrane potential, and mitochondrial DNA copy number through AMPK/ PKC/ NADPH oxidase signaling. Besides, DAPA reversed the H/R-induced apoptosis. Furthermore, we demonstrated that DAPA improved the I/R-induced cardiac dysfunction by echocardiography and abrogated the I/R-elicited apoptosis in the myocardium of rats. Also, the administration of DAPA mitigated the production of myocardial infarction markers. In conclusion, our data suggested that DAPA treatment holds the potential to ameliorate the I/R-elicited oxidative stress and the following cardiac apoptosis via modulation of AMPK, which attenuates the cardiac dysfunction caused by I/R injury.