Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response

• Published in: Veterinary research (Paris) Vol. 51; no. 1; p. 145
• Main Authors: Zhou, Jin-Run, Liu, Jun-Hong, Li, Hong-Mei, Zhao, Yue, Cheng, Ziqiang, Hou, Yan-Meng, Guo, Hui-Jun
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 09.12.2020; BioMed Central; BMC
• Subjects: Analysis; Antigens; antiviral bioactivities; Biological response modifiers; chicken TRIM25; Cloning; Genes; Interferon; Life Sciences; Poultry; Proteins; RNA; Spleen; subgroup A of avian leukosis virus; type I interferon response; Viral infections; Viruses; Beijing China; United States > US; China; subgroup A of avian leukosis virus; antiviral bioactivities; type I interferon response; chicken TRIM25
• ISSN: 1297-9716; 0928-4249; 1297-9716
• DOI: 10.1186/s13567-020-00870-1

This study focuses on the immunoregulatory effects of chicken TRIM25 on the replication of subgroup A of avian leukosis virus (ALV-A) and the MDA5-mediated type I interferon response. The ALV-A-SDAU09C1 strain was inoculated into DF1 cells and 1-day-old SPF chickens, and the expression of TRIM25 was detected at different time points after inoculation. A recombinant overexpression plasmid containing the chicken TRIM25 gene (TRIM25-GFP) was constructed and transfected into DF1 cells to analyse the effects of the overexpression of chicken TRIM25 on the replication of ALV-A and the expression of MDA5, MAVS and IFN-β. A small interfering RNA targeting chicken TRIM25 (TRIM25-siRNA) was prepared and transfected into DF1 cells to assess the effects of the knockdown of chicken TRIM25 on the replication of ALV-A and the expression of MDA5, MAVS and IFN-β. The results showed that chicken TRIM25 was significantly upregulated at all time points both in ALV-A-infected cells and in ALV-A-infected chickens. Overexpression of chicken TRIM25 in DF1 cells dramatically decreased the antigenic titres of ALV-A in the cell supernatant and upregulated the relative expression of MDA5, MAVS and IFN-β induced by ALV-A or by poly(I:C); in contrast, knockdown of chicken TRIM25 significantly increased the antigenic titres of ALV-A and downregulated the relative expression of MDA5, MAVS and IFN-β. It can be concluded that chicken TRIM25 can inhibit the replication of ALV-A and upregulate the MDA5 receptor-mediated type I interferon response in chickens. This study can help improve the understanding of the antiviral activities of chicken TRIM25 and enrich the knowledge of antiviral responses in chickens.