Microbial and metabolomic profiles in correlation with depression and anxiety co-morbidities in diarrhoea-predominant IBS patients

• Published in: BMC microbiology Vol. 20; no. 1; pp. 168 - 14
• Main Authors: Liu, Tong, Gu, Xiang, Li, Li-Xiang, Li, Ming, Li, Bing, Cui, Xiao, Zuo, Xiu-li
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 17.06.2020; BioMed Central; BMC
• Subjects: Abundance; Adult; Analysis; Anxiety; Anxiety - metabolism; Anxiety - microbiology; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Case-Control Studies; Comorbidity; Correlation analysis; Depression; Depression (Mood disorder); Depression - metabolism; Depression - microbiology; Diarrhea; Diarrhea - metabolism; Diarrhea - microbiology; Diarrhea - psychology; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fecal microflora; Feces - microbiology; Female; Gene sequencing; Glutarates - urine; Homoserine - analogs & derivatives; Homoserine - urine; Humans; Inositol; Intestinal microflora; Intestine; Irritable bowel syndrome; Irritable Bowel Syndrome - metabolism; Irritable Bowel Syndrome - microbiology; Irritable Bowel Syndrome - psychology; L-Serine; Male; Mental depression; Mental disorders; Metabolism; Metabolites; Metabolomics; Metabolomics - methods; Microbial community; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Middle Aged; Phylogeny; Population; Relative abundance; Resveratrol; RNA; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA - methods; Urine - chemistry; Urine - microbiology; Xanthines - urine; Metabolomics; Depression; Anxiety; Microbial community; Irritable bowel syndrome
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-020-01841-4

Psychological co-morbidities in irritable bowel syndrome (IBS) have been widely recognized, whereas less is known regarding the role of gut microbial and host metabolic changes in clinical and psychological symptoms in IBS. A total of 70 diarrhoea-predominant IBS (IBS-D) patients and 46 healthy controls were enrolled in this study. Stool and urine samples were collected from both groups for 16S rRNA gene sequencing and metabolomic analysis. The results showed that fecal microbiota in IBS-D featured depleted Faecalibacterium (adjusted P = 0.034), Eubacterium rectale group (adjusted P = 0.048), Subdoligranulum (adjusted P = 0.041) and increased Prevotella (adjusted P = 0.041). O-ureido-L-serine, 3,4-dihydroxybenzenesulfonic acid and (R)-2-Hydroxyglutarate demonstrated lower urinary concentrations in IBS-D patients. We further built correlation matrices between gut microbe abundance, differentiated metabolite quantities and clinical parameters. Dialister manifested negative association with IBS severity (r = - 0.285, P = 0.017), anxiety (r = - 0.347, P = 0.003) and depression level (r = - 0.308, P = 0.010). Roseburia was negatively associated with IBS severity (r = - 0.298, P = 0.012). Twenty metabolites correlated with anxiety or depression levels, including 3,4-dihydroxymandelaldehyde with SAS (r = - 0.383, P = 0.001), 1-methylxanthine with SDS (r = - 0.347, P = 0.004) and 1D-chiro-inositol with SAS (r = - 0.336, P = 0.005). In analysis of microbe-metabolite relationship, 3,4-dihydroxymandelaldehyde and 1-methylxanthine were negatively correlated with relative abundance of Clostridiumsensu stricto. Our findings demonstrated altered microbial and metabolomic profiles associated with clinically and psychological symptoms in IBS-D patients, which may provide insights for further investigations.