Adhesion of Staphylococcus aureus to Corneocytes from Atopic Dermatitis Patients Is Controlled by Natural Moisturizing Factor Levels

• Published in: mBio Vol. 9; no. 4; pp. e01184 - 18
• Main Authors: Feuillie, Cécile, Vitry, Pauline, McAleer, Maeve A, Kezic, Sanja, Irvine, Alan D, Geoghegan, Joan A, Dufrêne, Yves F
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 14.08.2018
• Subjects: Adhesins, Bacterial - metabolism; adhesion; atopic dermatitis; Bacterial Adhesion; Bacteriology; biofilms; Biomechanics; Dermatitis, Atopic - microbiology; Dermatitis, Atopic - pathology; Dermatology; Human health and pathology; Humans; Infectious diseases; Intermediate Filament Proteins - metabolism; Keratinocytes - microbiology; Life Sciences; Mechanics; Microbiology and Parasitology; Physics; Protein Binding; skin; Skin - microbiology; Staphylococcal Infections - microbiology; Staphylococcus aureus; Staphylococcus aureus - physiology; skin; atopic dermatitis; adhesion; biofilms; Staphylococcus aureus
• ISSN: 2161-2129; 2150-7511
• DOI: 10.1128/mbio.01184-18

The bacterial pathogen plays an important role in atopic dermatitis (AD), a chronic disorder that mostly affects children. Colonization of the skin of AD patients by exacerbates the disease, but the molecular determinants of the bacterium-skin adhesive interactions are poorly understood. Specifically, reduced levels of natural moisturizing factor (NMF) in the stratum corneum have been shown to be associated with more severe AD symptoms, but whether this is directly related to adhesion is still an open question. Here, we demonstrate a novel relationship between NMF expression in AD skin and strength of bacterial adhesion. Low-NMF corneocytes, unlike high-NMF ones, are covered by a dense layer of nanoscale villus protrusions. bacteria isolated from AD skin bind much more strongly to corneocytes when the NMF level is reduced. Strong binding forces originate from a specific interaction between the bacterial adhesion clumping factor B (ClfB) and skin ligands. Remarkably, mechanical tension dramatically strengthens ClfB-mediated adhesion, as observed with catch bonds, demonstrating that physical stress plays a role in promoting colonization of AD skin by Collectively, our findings demonstrate that patient NMF levels regulate the strength of -corneocyte adhesion, the first step in skin colonization, and suggest that the ClfB binding mechanism could represent a potential target for new therapeutic treatments. Bacterium-skin interactions play important roles in skin disorders, yet their molecular details are poorly understood. In this study, we decipher the molecular forces at play during adhesion of to skin corneocytes in the clinically important context of atopic dermatitis (AD), also known as eczema. We identify a unique relationship between the level of natural moisturizing factor (NMF) in the skin and the strength of bacterium-corneocyte adhesion. Bacterial adhesion is primarily mediated by the surface protein clumping factor B (ClfB) and is enhanced by physical stress, highlighting the role of protein mechanobiology in skin colonization. Similar to a catch bond behavior, this mechanism represents a promising target for the development of novel antistaphylococcal agents.