TMT-based quantitative proteomic analysis reveals defense mechanism of wheat against the crown rot pathogen Fusarium pseudograminearum

• Published in: BMC plant biology Vol. 21; no. 1; p. 82
• Main Authors: Qiao, Fangfang, Yang, Xiwen, Xu, Fengdan, Huang, Yuan, Zhang, Jiemei, Song, Miao, Zhou, Sumei, Zhang, Meng, He, Dexian
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 08.02.2021; BioMed Central; BMC
• Subjects: Acids; Agricultural research; Barley; Biosynthesis; Crown rot; Cultivars; Defense mechanism; Defense mechanisms; Differentially expressed proteins (DEPs); Disease; Disease Resistance - genetics; Diseases and pests; Edible Grain - genetics; Edible Grain - microbiology; Encyclopedias; Fungi, Phytopathogenic; Fusarium; Fusarium - pathogenicity; Fusarium pseudograminearum; Genes; Genetic aspects; Genetic Variation; Genomes; Genotype; Glutathione; Infections; Inoculation; Linolenic acid; Malondialdehyde; MAP kinase; Metabolism; Metabolites; Methods; Molecular modelling; Network analysis; Pathogens; Peptides; Photosynthesis; Physiological aspects; Physiology; Plant Diseases - genetics; Plant immunology; Plant-pathogen relationships; Polymerase chain reaction; Protein interaction; Protein-protein interactions; Proteins; Proteomics; Roots; Signal transduction; TMT; Triticum - genetics; Triticum - growth & development; Triticum - microbiology; Wheat; Wheat (Triticum aestivum L.); China; Differentially expressed proteins (DEPs); Fusarium pseudograminearum; Wheat (Triticum aestivum L.); Defense mechanism; TMT; Crown rot
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-021-02853-6

Fusarium crown rot is major disease in wheat. However, the wheat defense mechanisms against this disease remain poorly understood. Using tandem mass tag (TMT) quantitative proteomics, we evaluated a disease-susceptible (UC1110) and a disease-tolerant (PI610750) wheat cultivar inoculated with Fusarium pseudograminearum WZ-8A. The morphological and physiological results showed that the average root diameter and malondialdehyde content in the roots of PI610750 decreased 3 days post-inoculation (dpi), while the average number of root tips increased. Root vigor was significantly increased in both cultivars, indicating that the morphological, physiological, and biochemical responses of the roots to disease differed between the two cultivars. TMT analysis showed that 366 differentially expressed proteins (DEPs) were identified by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment in the two comparison groups, UC1110_3dpi/UC1110_0dpi (163) and PI610750_3dpi/PI610750_0dpi (203). It may be concluded that phenylpropanoid biosynthesis (8), secondary metabolite biosynthesis (12), linolenic acid metabolites (5), glutathione metabolism (8), plant hormone signal transduction (3), MAPK signaling pathway-plant (4), and photosynthesis (12) contributed to the defense mechanisms in wheat. Protein-protein interaction network analysis showed that the DEPs interacted in both sugar metabolism and photosynthesis pathways. Sixteen genes were validated by real-time quantitative polymerase chain reaction and were found to be consistent with the proteomics data. The results provided insight into the molecular mechanisms of the interaction between wheat and F. pseudograminearum.