Effect of cycloheximide on in vitro development of electrically activated feline oocytes

• Published in: Journal of Reproduction and Development Vol. 51; no. 6; pp. 783 - 786
• Main Authors: Karja, N.W.K.(Yamaguchi Univ. (Japan). Faculty of Agriculture), Otoi, T, Murakami, M, Wongsrikeao, P, Budiyanto, A, Fahrudin, M, Nagai, T
• Format: Journal Article
• Language: English
• Published: Japan THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 01.12.2005
• Subjects: Animals; Blastocyst - cytology; Cat; CATS; Cells, Cultured; CHAT; CICLOHEXIMIDA; CULTIVO IN VITRO; CULTURE IN VITRO; CYCLOHEXIMIDE; Cycloheximide - pharmacology; DESARROLLO EMBRIONARIO; DEVELOPPEMENT EMBRYONNAIRE; Electric field strength; Electric Stimulation; EMBRYONIC DEVELOPMENT; ESTIMULO; Female; GATO; IN VITRO CULTURE; In Vitro Techniques; NOYAU CELLULAIRE; Nuclear transfer; Nuclear Transfer Techniques; NUCLEO; NUCLEUS; Oocytes - cytology; Oocytes - drug effects; Oocytes - physiology; OVA; OVULE; OVULO; Parthenogenesis - drug effects; Parthenogenesis - physiology; Parthenogenetic activation; Protein Synthesis Inhibitors - pharmacology; REPIQUAGE; STIMULI; STIMULUS; TRANSPLANTING; TRASPLANTE
• ISSN: 0916-8818; 1348-4400
• DOI: 10.1262/jrd.17056

This study was conducted to improve parthenogenetic development in vitro of feline oocytes following a combined activation treatment of electrical stimulation and cycloheximide. In vitro matured (IVM) oocytes were stimulated electrically by a DC electrical pulse of 2 kV/cm for 50 mus. The stimulated oocytes were then incubated in MK-1 medium with or without cycloheximide and subsequently cultured in vitro for 6 days. No significant differences were observed between the two groups with respect to the proportions of cleavage, development to the morula stage, and the cell number of blastocysts. However, exposure of electrically stimulated oocytes to cycloheximide significantly increased the rate of development of the stimulated oocytes into the blastocyst stage compared with oocytes stimulated by electrical stimulation alone (31.0% vs 6.7%). The results from the present study suggested that a single electrical stimulation was insufficient to activate the IVM cat oocytes at 24 h of maturation and that exposure to cycloheximide following electrical stimulation improved the efficacy of the parthenogenetic development of domestic cat oocytes.