Identification of a new effector-immunity pair of Aeromonas hydrophila type VI secretion system

• Published in: Veterinary research (Paris) Vol. 51; no. 1; pp. 71 - 14
• Main Authors: Ma, Shuiyan, Dong, Yuhao, Wang, Nannan, Liu, Jin, Lu, Chengping, Liu, Yongjie
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 24.05.2020; BioMed Central; BMC
• Subjects: Aeromonas hydrophila - genetics; Aeromonas hydrophila - pathogenicity; Aeromonas hydrophila - physiology; Animals; Antibiotics; Bacteria; Bacterial Proteins - immunology; Biofilms; Bioinformatics; Competition; Deoxyribonucleic acid; DNA; E coli; Experiments; Fish Diseases - immunology; Fish Diseases - microbiology; Fisheries; Genes; Genes, Bacterial - immunology; Glycine; Gram-negative bacteria; Gram-Negative Bacterial Infections - immunology; Gram-Negative Bacterial Infections - microbiology; Gram-Negative Bacterial Infections - veterinary; Life Sciences; Lipase; Microbial Interactions; Phylogeny; Plasmids; Proteins; Type VI Secretion Systems - immunology; Virulence; Zebrafish; China; United States > US
• ISSN: 1297-9716; 0928-4249; 1297-9716
• DOI: 10.1186/s13567-020-00794-w

The type VI secretion system (T6SS) is a multiprotein weapon that kills eukaryotic predators or prokaryotic competitors by delivering toxic effectors. Despite the importance of T6SS in bacterial environmental adaptation, it is still challenging to systematically identify T6SS effectors because of their high diversity and lack of conserved domains. In this report, we discovered a putative effector gene, U876-17730, in the whole genome of Aeromonas hydrophila NJ-35 based on the reported conservative domain DUF4123 (domain of unknown function), with two cognate immunity proteins encoded downstream. Phylogenetic tree analysis of amino acids indicates that AH17730 belongs to the Tle1 (type VI lipase effector) family, and therefore was named Tle1 . The deletion of tle1 resulted in significantly decreased biofilm formation, antibacterial competition ability and virulence in zebrafish (Danio rerio) when compared to the wild-type strain. Only when the two immunity proteins coexist can bacteria protect themselves from the toxicity of Tle1 . Further study shows that Tle1 is a kind of phospholipase that possesses a conserved lipase motif, Gly-X-Ser-X-Gly (X is for any amino acid). Tle1 is secreted by T6SS, and this secretion requires its interaction with an associated VgrG (valine-glycine repeat protein G). In conclusion, we identified a T6SS effector-immunity pair and verified its function, which lays the foundation for future research on the role of T6SS in the pathogenic mechanism of A. hydrophila.