Consequences for the bovine embryo of being derived from a spermatozoon subjected to post-ejaculatory aging and heat shock: Development to the blastocyst stage and sex ratio

The objective was to determine whether aging of sperm caused by incubation at normothermic (38.5 C) or heat shock (40 C) temperatures for 4 h prior to oocyte insemination affects sperm motility, fertilizing ability, competence of the resultant embryo to develop to the blastocyst stage and blastocyst...

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Bibliographic Details
Published inJournal of Reproduction and Development Vol. 55; no. 1; pp. 69 - 74
Main Authors Hendricks, K.E.M.(University of Florida, Gainesville (USA)), Martins, L, Hansen, P.J
Format Journal Article
LanguageEnglish
Japanese
Published Japan THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 01.02.2009
Japan Science and Technology Agency
Subjects
AGING
Animals
Blastocyst - physiology
Blastocysts
BOVIN
Bovine
CATTLE
Cattle - embryology
Cells, Cultured
Cellular Senescence - physiology
CHOC
Cleavage Stage, Ovum - physiology
DESARROLLO EMBRIONARIO
Developmental stages
DEVELOPPEMENT EMBRYONNAIRE
Ejaculation
Embryo, Mammalian
EMBRYONIC DEVELOPMENT
Embryos
ENVEJECIMIENTO
ESPERMATOZOO
ESTRES TERMICO
Experiments
FECONDATION
FECUNDACION
Female
FERTILIZATION
GANADO BOVINO
Heat shock
HEAT STRESS
Hot Temperature - adverse effects
Hyperthermia
Male
Motility
Oocytes
Pregnancy
Sex Ratio
SHOCK
Sperm
Sperm Motility - physiology
SPERMATOZOA
Spermatozoa - physiology
SPERMATOZOIDE
STRESS THERMIQUE
Stress, Physiological - physiology
TEMPERATURA
TEMPERATURE
VIEILLISSEMENT
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Summary:The objective was to determine whether aging of sperm caused by incubation at normothermic (38.5 C) or heat shock (40 C) temperatures for 4 h prior to oocyte insemination affects sperm motility, fertilizing ability, competence of the resultant embryo to develop to the blastocyst stage and blastocyst sex ratio. In the first experiment, the percent of sperm that were motile was reduced by aging (P0.001) and the reduction in motility was greater for sperm at 40 C compared to sperm at 38.5 C (P0.01). In the second experiment, oocytes were inseminated with aged sperm. A smaller percent of oocytes fertilized with sperm aged at either temperature cleaved by Day 3 after insemination than oocytes fertilized with fresh sperm (P0.05). There was no effect of sperm aging on the percent of oocytes or cleaved embryos that developed to the blastocyst stage. Aging of sperm before fertilization at 38.5 C reduced the percent of blastocysts that were male (P
Bibliography:2009001215
L53
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.20097