Association of DNA repair genes polymorphisms with childhood acute lymphoblastic leukemia: a high-resolution melting analysis

• Published in: BMC research notes Vol. 15; no. 1; p. 46
• Main Authors: Zehtab, Shahrzad, Sattarzadeh Bardsiri, Mahla, Mirzaee Khalilabadi, Roohollah, Ehsan, Mohsen, Fatemi, Ahmad
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 14.02.2022; BioMed Central; BMC
• Subjects: Acute lymphoblastic leukemia; Acute lymphocytic leukemia; Amino acids; Blood; Case-Control Studies; Cell Cycle Proteins - genetics; Child; Childhood acute lymphoblastic Leukemia; Children; Deoxyribonucleic acid; DNA; DNA damage; DNA repair; DNA Repair - genetics; DNA-Binding Proteins - genetics; Genes; Genetic analysis; Genetic aspects; Genetic polymorphisms; Genetic Predisposition to Disease; Genetic testing; Genotype; Genotype & phenotype; Genotyping; High-resolution melting analysis; Humans; Iran; Leukemia; Leukemia in children; Leukemia research; Lymphatic leukemia; Lymphoblastic leukemia in children; Lymphocytic leukemia in children; Medical prognosis; NBN; Nuclear Proteins - genetics; Polymorphism, Single Nucleotide; Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics; Proteins; Research Note; Risk factors; Single nucleotide variants; Software; X-ray Repair Cross Complementing Protein 1 - genetics; XRCC1; XRCC1 protein; Iran; XRCC1; Childhood acute lymphoblastic Leukemia; NBN; Single nucleotide variants; High-resolution melting analysis
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-022-05918-3

Acute lymphoblastic leukemia (ALL) is one of the most common cancers in children for which the exact pathogenesis is not yet known. Single-nucleotide variants (SNVs) in different DNA repair genes are reported to be associated with ALL risk. This study aimed to determine the association between XRCC1 (rs1799782) and NBN (rs1805794, rs709816) SNVs and childhood ALL risk in a sample of the Iranian population. Fifty children with ALL and 50 age- and sex-matched healthy children were included in this case-control study. Genotyping of the mentioned SNVs was done by high-resolution melting (HRM) analysis. The prevalence of all three SNVs in XRCC1 and NBN genes did not differ between the patient and control groups, and these polymorphisms were not associated with childhood ALL risk (P > 0.05). HRM was a practical method for the detection of SNVs in XRCC1 and NBN genes. We found no significant association between XRCC1 (rs1799782) and NBN (rs1805794, rs709816) SNVs and childhood ALL risk.