Three-dimensional printed hydroxyapatite bone tissue engineering scaffold with antibacterial and osteogenic ability

• Published in: Journal of biological engineering Vol. 15; no. 1; p. 21
• Main Authors: Zhongxing, Liu, Shaohong, Wu, Jinlong, Li, Limin, Zhang, Yuanzheng, Wang, Haipeng, Gao, Jian, Cao
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 09.08.2021; BioMed Central; BMC
• Subjects: 3D printed; Alkaline phosphatase; Antibacterial; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Antimicrobial peptides; Binding; Biological products; Biomaterials; Biomedical materials; Bone biomaterials; Bone growth; Bone morphogenetic protein 2; Bone morphogenetic proteins; Bone scaffold; Cbfa-1 protein; Cell proliferation; Collagen; Collagen (type I); E coli; Gene expression; Genetic translation; Growth factors; Hydroxyapatite; Mechanical properties; Methicillin; Osteocalcin; Osteoinduction; Peptides; Phosphatases; Political aspects; Polymers; Polypeptides; Proteins; Regeneration; Scaffolds; Scanning electron microscopy; Software; Staphylococcus infections; Three dimensional printing; Tissue engineering; China; Hydroxyapatite; Antibacterial; Bone scaffold; Osteoinduction; 3D printed
• ISSN: 1754-1611; 1754-1611
• DOI: 10.1186/s13036-021-00273-6

The development of an effective scaffold for bone defect repair is an urgent clinical need. However, it is challenging to design a scaffold with efficient osteoinduction and antimicrobial activity for regeneration of bone defect. In this study, we successfully prepared a hydroxyapatite (HA) porous scaffold with a surface-specific binding of peptides during osteoinduction and antimicrobial activity using a three-dimensional (3D) printing technology. The HA binding domain (HABD) was introduced to the C-terminal of bone morphogenetic protein 2 mimetic peptide (BMP2-MP) and antimicrobial peptide of PSI10. The binding capability results showed that BMP2-MP and PSI10-containing HABD were firmly bound to the surface of HA scaffolds. After BMP2-MP and PSI10 were bound to the scaffold surface, no negative effect was observed on cell proliferation and adhesion. The gene expression and protein translation levels of type I collagen (COL-I), osteocalcin (OCN) and Runx2 have been significantly improved in the BMP2-MP/HABP group. The level of alkaline phosphatase significantly increased in the BMP2-MP/HABP group. The inhibition zone test against Staphylococcus aureus and Escherichia coli BL21 prove that the PSI10/HABP@HA scaffold has strong antibacterial ability than another group. These findings suggest that 3D-printed HA scaffolds with efficient osteoinduction and antimicrobial activity represent a promising biomaterial for bone defect reconstruction.