Total RNA and genomic DNA of Lactobacillus gasseri OLL2809 induce interleukin-12 production in the mouse macrophage cell line J774.1 via toll-like receptors 7 and 9

• Published in: BMC microbiology Vol. 20; no. 1; p. 217
• Main Authors: Onishi, Kazumasa, Mochizuki, Junko, Sato, Asako, Goto, Ayako, Sashihara, Toshihiro
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 20.07.2020; BioMed Central; BMC
• Subjects: Bacteria; Biotechnology; Cytokines; Deoxyribonucleic acid; DNA; Genomic DNA; Genomics; IL-12; Immune system; Interleukin 12; Interleukins; Lactobacilli; Lactobacillus gasseri; Lactobacillus gasseri OLL2809; Ligands; Macrophages; MyD88 protein; Peptides; Phagocytosis; Receptors; Ribonucleic acid; RNA; Signal transduction; Toll-like receptors; Total RNA; Total RNA; IL-12; Lactobacillus gasseri OLL2809; Phagocytosis; Genomic DNA
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-020-01900-w

Lactobacillus gasseri OLL2809 can highly induce interleukin (IL)-12 production in immune cells. Even though beneficial properties of this strain for both humans and animals have been reported, the mechanism by which the bacteria induces the production of IL-12 in immune cells remains elusive. In this study, we investigated the mechanism of induction of IL-12 using a mouse macrophage cell line J774.1. Inhibition of phagocytosis of L. gasseri OLL2809, and myeloid differentiation factor 88 and Toll-like receptors (TLRs) 7 and 9 signalling attenuated IL-12 production in J774.1 cells. Total RNA and genomic DNA of L. gasseri OLL2809, when transferred to the J774.1 cells, also induced IL-12 production. The difference in the IL-12-inducing activity of Lactobacilli is attributed to the susceptibility to phagocytosis, but not to a difference in the total RNA and genomic DNA of each strain. We concluded that total RNA and genomic DNA of phagocytosed L. gasseri OLL2809 induce IL-12 production in J774.1 cell via TLRs 7 and 9, and the high IL-12-inducing activity of L. gasseri OLL2809 is due to its greater susceptibility to phagocytosis.