Downregulation of the ubiquitin ligase KBTBD8 prevented epithelial ovarian cancer progression

Kelch repeat and BTB domain-containing protein 8, KBTBD8, has been identified as a female fertility factor. However, there have been no reports on the role of KBTBD8 in the progression of epithelial ovarian cancer, EOC. Our study aimed to address this issue. We first examine KBTBD8 expression in EOC...

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Published inMolecular medicine (Cambridge, Mass.) Vol. 26; no. 1; p. 96
Main Authors Du, Lei, Li, Cong-Rong, He, Qi-Feng, Li, Xiao-Hua, Yang, Lin-Fei, Zou, Yuan, Yang, Zhi-Xia, Zhang, Dong, Xing, Xiao-Wei
Format Journal Article
LanguageEnglish
Published England BioMed Central 27.10.2020
BMC
Subjects
Breast cancer
Cell cycle
Cell growth
Epithelial ovarian cancer
Female fertility factor
Fertility
KBTBD8
Ovarian cancer
Plasmids
Proteins
Tumorigenesis
Tumors
Ubiquitin ligase
Wound healing
England
United States > US
China
Japan
Ubiquitin ligase
Epithelial ovarian cancer
KBTBD8
Female fertility factor
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Summary:Kelch repeat and BTB domain-containing protein 8, KBTBD8, has been identified as a female fertility factor. However, there have been no reports on the role of KBTBD8 in the progression of epithelial ovarian cancer, EOC. Our study aimed to address this issue. We first examine KBTBD8 expression in EOC tissues and cells. Next, we performed RNA sequencing to reveal the overall mechanism. Then we investigated the roles of KBTBD8 in the proliferation, migration, and health status of cultured EOC cells. Finally, we employed tumor xenograft models to evaluate the role of KBTBD8 in vivo. First, KBTBD8 level was significantly higher in EOC tissues and cells. Next, comparative RNA sequencing identified more tumorigenesis-related genes that KBTBD8 might regulate. Then we found that KBTBD8 knockdown significantly decreased EOC cell proliferation, migration, and the activities of multiple tumorigenesis-related kinases. Finally, KBTBD8 knockdown significantly diminished ovarian tumor formation in vivo. Proper KBTBD8 level is essential for the healthy growth of ovarian somatic cells, such as ovarian epithelial cells. Excessive KBTBD8 might be a significant impetus for EOC progression. KBTBD8 reduction greatly inhibits EOC proliferation and migration.
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ISSN:1076-1551
1528-3658
DOI:10.1186/s10020-020-00226-7