Activation of cortical somatostatin interneurons prevents the development of neuropathic pain

• Published in: Nature neuroscience Vol. 20; no. 8; pp. 1122 - 1132
• Main Authors: Cichon, Joseph, Blanck, Thomas J J, Gan, Wen-Biao, Yang, Guang
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.08.2017; Nature Publishing Group
• Subjects: 14/69; 631/378/1689/2610; 631/378/2620/2618; 631/378/3920; Action Potentials - physiology; Activation; Animal Genetics and Genomics; Animals; Behavioral Sciences; Biological Techniques; Biomedicine; Calcium; Calcium signalling; Chronic pain; Cortex (somatosensory); Dendrites; Dendrites - metabolism; Firing pattern; Glutamic acid receptors (ionotropic); Health aspects; Hyperactivity; Interneurons; Interneurons - physiology; Intestine; Methods; Mice, Transgenic; N-Methyl-D-aspartic acid receptors; Nerve Net - physiopathology; Neuralgia; Neuralgia - metabolism; Neuralgia - physiopathology; Neurobiology; Neurophysiology; Neurosciences; Pain; Pain management; Pain perception; Parvalbumin; Pharmacology; Prevention; Pyramidal Cells - physiology; Receptors, N-Methyl-D-Aspartate - metabolism; Rodents; Somatosensory Cortex - physiology; Somatosensory Cortex - physiopathology; Somatostatin; Somatostatin - metabolism; Vasoactive agents; Vasoactive intestinal peptide; Vasoactive Intestinal Peptide - metabolism; United States
• ISSN: 1097-6256; 1546-1726
• DOI: 10.1038/nn.4595

Using in vivo calcium imaging in a mouse model of neuropathic pain, the authors found a persistent increase in the activity of somatosensory cortex pyramidal neurons following peripheral nerve injury. Repeated pharmacogenetic activation of somatostatin-expressing inhibitory neurons after injury not only corrected this abnormal cortical activity but also prevented the development of chronic pain. Neuropathic pain involves long-lasting modifications of pain pathways that result in abnormal cortical activity. How cortical circuits are altered and contribute to the intense sensation associated with allodynia is unclear. Here we report a persistent elevation of layer V pyramidal neuron activity in the somatosensory cortex of a mouse model of neuropathic pain. This enhanced pyramidal neuron activity was caused in part by increases of synaptic activity and NMDA-receptor-dependent calcium spikes in apical tuft dendrites. Furthermore, local inhibitory interneuron networks shifted their activity in favor of pyramidal neuron hyperactivity: somatostatin-expressing and parvalbumin-expressing inhibitory neurons reduced their activity, whereas vasoactive intestinal polypeptide–expressing interneurons increased their activity. Pharmacogenetic activation of somatostatin-expressing cells reduced pyramidal neuron hyperactivity and reversed mechanical allodynia. These findings reveal cortical circuit changes that arise during the development of neuropathic pain and identify the activation of specific cortical interneurons as therapeutic targets for chronic pain treatment.