Inactivation of the T6SS inner membrane protein DotU results in severe attenuation and decreased pathogenicity of Aeromonas veronii TH0426

• Published in: BMC microbiology Vol. 20; no. 1; pp. 76 - 11
• Main Authors: Song, Haichao, Kang, Yuanhuan, Qian, Aidong, Shan, Xiaofeng, Li, Ying, Zhang, Lei, Zhang, Haipeng, Sun, Wuwen
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 03.04.2020; BioMed Central; BMC
• Subjects: Aeromonas; Aeromonas veronii; Aeromonas veronii - genetics; Aeromonas veronii - metabolism; Aeromonas veronii - pathogenicity; Animals; Attenuation; Bacteria; Bacterial proteins; Biofilms; Biofilms - growth & development; Cell adhesion & migration; Colonies; Deactivation; Deoxyribonucleic acid; Disease Models, Animal; DNA; dotU; E coli; Environmental changes; Gene deletion; Genetic aspects; Genomes; Gram-Negative Bacterial Infections - microbiology; Hydrogen-Ion Concentration; Inactivation; Infections; Infectivity; Invasiveness; Lethal Dose 50; Membrane proteins; Membranes; Morphology; Motility; Mutants; Mutation; Pathogenesis; Pathogenicity; Pathogens; Properties; Proteins; Proteomes; T6SS; Type VI Secretion Systems - genetics; Virulence; Virulence (Microbiology); Whole Genome Sequencing; Zebrafish; China; Pathogenicity; Aeromonas veronii; dotU; T6SS; Virulence
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-020-01743-5

The inner membrane protein DotU of Aeromonas veronii is an important component of the minimal core conserved membrane proteome required for the formation of an envelope-transmembrane complex. This protein functions in a type VI secretion system (T6SS), and the role of this T6SS during the pathogenic process has not been clearly described. A recombinant A. veronii with a partial disruption of the dotU gene (720 bp of the in-frame sequence) (defined as ∆dotU) was constructed by two conjugate exchanges. We found that the mutant ∆dotU allele can be stably inherited for more than 50 generations. Inactivation of the A. veronii dotU gene resulted in no significant changes in growth or resistance to various environmental changes. However, compared with the wild-type strain colony, the mutant ∆dotU colony had a rough surface morphology. In addition, the biofilm formation ability of the mutant ∆dotU was significantly enhanced by 2.1-fold. Conversely, the deletion of the dotU gene resulted in a significant decrease in pathogenicity and infectivity compared to those of the A. veronii wild-type strain. Our findings indicated that the dotU gene was an essential participant in the pathogenicity and invasiveness of A. veronii TH0426, which provides a novel perspective on the pathogenesis of TH0426 and lays the foundation for discovering potential T6SS effectors.