Hepatocellular Carcinoma LINC01116 Outcompetes T Cells for Linoleic Acid and Accelerates Tumor Progression

• Published in: Advanced science Vol. 11; no. 21; pp. e2400676 - n/a
• Main Authors: Ma, Kun, Chu, Junhui, Liu, Yufeng, Sun, Linmao, Zhou, Shuo, Li, Xianying, Ji, Changyong, Zhang, Ning, Guo, Xinyu, Liang, Shuhang, Cui, Tianming, Hu, Qingsong, Wang, Jiabei, Liu, Yao, Liu, Lianxin
• Format: Journal Article
• Language: English
• Published: Germany John Wiley & Sons, Inc 01.06.2024; John Wiley and Sons Inc; Wiley
• Subjects: Animals; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - immunology; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Cell Line, Tumor; Cloning; Disease Models, Animal; Disease Progression; EWSR1; Experiments; Fatty acids; Gene Expression Regulation, Neoplastic - genetics; Genes; hepatocellular carcinoma; Humans; Immunotherapy; LINC01116; Linoleic Acid - metabolism; lipid metabolism; Lipids; Liver cancer; Liver Neoplasms - genetics; Liver Neoplasms - immunology; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Localization; Lymphocytes; Medical prognosis; Metabolism; Metabolites; Metastasis; Mice; Oxidation; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; Tumor Microenvironment - genetics; Tumor Microenvironment - immunology; EWSR1; hepatocellular carcinoma; immunotherapy; lipid metabolism; LINC01116
• ISSN: 2198-3844; 2198-3844
• DOI: 10.1002/advs.202400676

Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer with a highly immunosuppressive tumor microenvironment and a typical pattern of disturbances in hepatic lipid metabolism. Long non‐coding RNAs are shown to play an important role in the regulation of gene expression, but much remains unknown between tumor microenvironment and lipid metabolism as a bridging molecule. Here, long intergenic nonprotein coding RNA 01116 (LINC01116) acts as this molecular which is frequently upregulated in HCC patients and associated with HCC progression in vitro and in vivo is identified. Mechanistically, LINC01116 stabilizes EWS RNA‐binding protein 1 (EWSR1) by preventing RAD18 E3 Ubiquitin Protein Ligase (RAD18) ‐mediated ubiquitination. The enhanced EWSR1 protein upregulates peroxisome proliferator activated receptor alpha (PPARA) and fatty acid binding protein1 (FABP1) expression, a long‐chain fatty acid (LCFA) transporter, and thus cancer cells outcompete T cells for LCFAs, especially linoleic acid, for seeding their own growth, leading to T cell malfunction and HCC malignant progression. In a preclinical animal model, the blockade of LINC01116 leads to enhanced efficacy of anti‐PD1 treatment accompanied by increased cytotoxic T cell and decreased exhausted T cell infiltration. Collectively, LINC01116 is an immunometabolic lncRNA and the LINC01116‐EWSR1‐PPARA‐FABP1 axis may be targetable for cancer immunotherapy. LINC01116, an immunometabolic lncRNA, promoted its own growth through competitive consumption of linoleic acid and attenuated the efficacy of immunotherapy is identified.