In vitro response to alkaline phosphatase coatings immobilized onto titanium implants using electrospray deposition or polydopamine-assisted deposition

• Published in: Journal of biomedical materials research. Part A Vol. 102; no. 4; pp. 1102 - 1109
• Main Authors: Nijhuis, Arnold W. G., van den Beucken, Jeroen J.J.P., Jansen, John A., Leeuwenburgh, Sander C. G.
• Format: Journal Article
• Language: English
• Published: Hoboken, NJ Blackwell Publishing Ltd 01.04.2014; Wiley-Blackwell; Wiley Subscription Services, Inc
• Subjects: Alkaline phosphatase; Alkaline Phosphatase - pharmacology; Animals; Biocompatibility; Biological and medical sciences; Biomedical materials; Calcium - metabolism; Cattle; Cell Adhesion - drug effects; Cell Differentiation - drug effects; Cell Movement - drug effects; Cell Proliferation - drug effects; Cell Shape - drug effects; Cells, Cultured; Coated Materials, Biocompatible - pharmacology; Coatings; Deposition; Differentiation; DNA - metabolism; enzyme immobilization; Enzymes, Immobilized - pharmacology; Extracellular Matrix - drug effects; Extracellular Matrix - metabolism; Immobilization; Indoles - chemistry; Male; Materials Testing - methods; Medical sciences; Mineralization; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - metabolism; Osteoblasts - ultrastructure; polydopamine; Polymers - chemistry; Prostheses and Implants; Rats; Rats, Wistar; surface modification; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgical implants; Technology. Biomaterials. Equipments; Titanium; Titanium - pharmacology; titanium implants; Water - chemistry; Alkaline phosphatase; Coating material; Implant; Enzyme; surface modification; Phosphoric monoester hydrolases; Immobilization; Polymer; Esterases; Coatings; In vitro; Electrospray; Surface treatment; titanium implants; polydopamine; Amine polymer; Titanium; enzyme immobilization; Hydrolases; Biomaterial; Immobilized enzyme; Biomedical engineering; alkaline phosphatase
• ISSN: 1549-3296; 1552-4965; 1552-4965
• DOI: 10.1002/jbm.a.34776

Immobilization of biomolecules onto implant surfaces is one of the most straightforward strategies to control the interaction between an implant and its biological environment. Recently, it was shown that the enzyme alkaline phosphatase (ALP) could be efficiently immobilized onto titanium implants in a single step using polydopamine. We hypothesized that such polydopamine‐ALP coatings can enhance the early attachment of cells and increase mineralization. Therefore, the current study aimed at immobilization of ALP onto titanium by means of either one‐ or two‐step polydopamine‐assisted immobilization or electrospray deposition, the comparative characterization of these experimental substrates and subsequent cell behavioral analysis using primary osteoblast‐like cells. Uncoated titanium and ALP‐free polydopamine coatings served as controls. Despite significant ALP surface activity and lower water contact for angles ALP‐containing surface modifications, only marginal effects on early cell behavior (i.e., cell spreading) and osteogenic differentiation (i.e., proliferation, differentiation and mineralization) were observed in comparison to uncoated titanium. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1102–1109, 2014.