Transcriptional regulation of lipid homeostasis in mycobacteria

• Published in: Molecular microbiology Vol. 78; no. 1; pp. 64 - 77
• Main Authors: Salzman, V, Mondino, S, Sala, C, Cole, S.T, Gago, G, Gramajo, H
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.10.2010; Blackwell Publishing Ltd; Blackwell
• Subjects: Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Binding Sites; Biochemistry; Biological and medical sciences; Enzymes; Fatty Acid Synthase, Type II - genetics; Fatty Acid Synthase, Type II - metabolism; Fatty acids; Fatty Acids - biosynthesis; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Bacterial; Genes, Regulator; Homeostasis; Lipid Metabolism; Microbiology; Miscellaneous; Mutation; Mycobacterium smegmatis; Mycobacterium smegmatis - enzymology; Mycobacterium smegmatis - genetics; Mycobacterium tuberculosis; Operon; Promoter Regions, Genetic; Mycobacterium; Regulation(control); Transcription; Mycobacteriales; Mycobacteriaceae; Homeostasis; Bacteria; Lipids; Actinomycetes
• ISSN: 0950-382X; 1365-2958
• DOI: 10.1111/j.1365-2958.2010.07313.x

Mycolic acids are major components of the cell envelope of mycobacteria, such as Mycobacterium tuberculosis, and play an important role in its architecture, impermeability and interaction with the environment. Synthesis of mycolic acids is carried out by two types of fatty acid synthases (FAS) working in concert: type I FAS, a multifunctional enzyme capable of de novo synthesis of medium-chain fatty acids, and type II FAS, responsible for their elongation. In this article we report the identification and characterization of a transcriptional regulator (MabR), whose binding to the FAS-II promoter region was demonstrated in vitro and in vivo. Overexpression and knock-down studies in Mycobacterium smegmatis revealed the repressor nature of MabR, with reduced amounts of FAS-II transcripts and fatty acids in the overproducing strain. Under these conditions, downregulation of fas transcription was also observed, thereby suggesting the existence of cross-talk between the two FAS, mediated by MabR. Finally, the finding that a mabR knock-out mutant could only be obtained in a merodiploid strain of M. smegmatis, confirmed the predicted essentiality, thus implying an essential role for MabR in mycobacterial fatty acid metabolism.