笹之雪组织培养和植株再生体系研究

通过对笹之雪（Agave victoriaereginae）不同外植体、不同激素配比的研究,建立笹之雪离体培养高效植株再生体系.笹之雪侧芽在含2.0mg·L^-1 6-BA和0.2mg·L^-1 NAA的MS培养基中增殖效果较好,增殖系数4.6.比较分析叶片各部位愈伤组织诱导和再生能力,结果表明：叶基＞叶中＞叶顶,且只有叶基诱导出有效愈伤组织,是较好的外植体材料;叶片各部位在2.0mg·L^-16-BA、1.0mg·L^-1KT和0.2mg·L^-1 NAA的MS培养基上愈伤组织的诱导率达到最高,叶基、叶中和叶顶诱导率分别为100％、98％、89％,大部分叶基还能通过愈伤组织间接分化形成不定芽...

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Published in西南农业学报 Vol. 27; no. 5; pp. 2145 - 2150
Main Author 吕平 韦丽君 金刚 庞新华
Format Journal Article
LanguageChinese
Published 广西壮族自治区亚热带作物研究所,广西南宁530001 2014
广西亚热带优势作物繁育及综合开发新技术重点实验室,广西南宁530001%广西壮族自治区亚热带作物研究所,广西南宁,530001
Subjects
愈伤组织
植株再生
笹之雪
组织培养
笹之雪
Callus
组织培养
Plant regenerating
Tissue culture
愈伤组织
植株再生
Agave victoriaereginae
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ISSN1001-4829

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Summary:通过对笹之雪（Agave victoriaereginae）不同外植体、不同激素配比的研究,建立笹之雪离体培养高效植株再生体系.笹之雪侧芽在含2.0mg·L^-1 6-BA和0.2mg·L^-1 NAA的MS培养基中增殖效果较好,增殖系数4.6.比较分析叶片各部位愈伤组织诱导和再生能力,结果表明：叶基＞叶中＞叶顶,且只有叶基诱导出有效愈伤组织,是较好的外植体材料;叶片各部位在2.0mg·L^-16-BA、1.0mg·L^-1KT和0.2mg·L^-1 NAA的MS培养基上愈伤组织的诱导率达到最高,叶基、叶中和叶顶诱导率分别为100％、98％、89％,大部分叶基还能通过愈伤组织间接分化形成不定芽.将有效愈伤组织转至分化培养基上,在MS＋6-BA 1.0 mg·L^-1＋ KT1.5 mg·L^-1＋ NAA0.2mg· L^-1培养基上的总分化芽数最多,长势也较其他配方的好.MS＋6-BA 0.5 mg·L^-1＋KT 1.0 mg·L^-1＋ NAA 0.2 mg·L^-1对不定芽的增殖效果最好,增殖率100％,平均增殖系数5.5.在不定芽生根中,以MS＋KT1.5 mg·L^-1＋ NAA 0.5 mg·L^-1效果较好,出根率高,根系粗壮.
Bibliography:51-1213/S
LV Ping,WEI Li-jun,JIN Gang,PANG Xin-hua（Guangxi Institute of Subtropical Crops, Guangxi Nanning 530001, China;Guangxi Subtropical Key Laboratory of Advantage Crops Breeding and New Technological Development, Guangxi Nanning 530001, China）
Different Agave victoriaereginae explants and the compounding proportions of different hormones were comparatively studied in tissue culture and thereafter a high-efficient plant-regeneration system by Agate victoriaereginaein vitro culture was established.The medium MS ＋ 6-BA 2.0 mg· L-1 ＋ NAA 0.2 mg · L-1 was the best to multiply shoot from lateral bud of Agate victoriaereginae,with multiplication coefficient of 4.6.The sequence of callus induction and regeneration of different places in 1eaf was leaf base ＞ leaf mid ＞ leaf top.Effective callus was ouly induced from leaf base and they were great explants materials.The induction medium MS ＋ 6-BA 2.0mg · L-1 ＋ 1.0 KT mg · L-1 ＋ NAA 0.2 mg · L-1 was the best to induce callus from different places in 1eaf and leaf bas
ISSN:1001-4829