Micro-CT对急性肺结核小鼠模型动态演变的监测价值

目的：探讨micro-CT对急性肺结核小鼠动态演变过程的监测价值。方法：用滴鼻法建立急性感染C57BL/6J小鼠肺结核模型,设为实验组,同时设立对照组。两组分别在实验组滴鼻后第1、4、8、12周进行micro-CT扫描,并与病理学检查结果对比。结果：实验组共40只小鼠,死亡9只,3只肺内阴性,余28只小鼠分为4个亚组（分别7、6、8、7只）。对照组4亚组,每组5只。实验组小鼠感染第1周,71.4%表现为磨玻璃密度影（GGO）,57.1%表现为支气管充气征,对应病理表现为肺泡腔渗出和肺泡间隔增宽;感染第4周,micro-CT主要表现为GGO和小灶性实变（66.7%）,对应病理表现为肺泡腔渗出和实...

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Published in中国临床医学 Vol. 24; no. 4; pp. 531 - 535
Main Author 王青乐 张志勇 施裕新 朱召芹 冯艳玲 张兴伟 冯峰 张昊凌 曾蒙苏 陈刚
Format Journal Article
LanguageChinese
Published 复旦大学附属中山医院放射科,上海市影像医学研究所,复旦大学上海医学院影像医学系,上海 200032%复旦大学附属公共卫生临床中心,上海,201508%南通市肿瘤医院影像科,南通,226361 2017
Subjects
X线计算机体层摄影术
动物模型
病理学
肺结核
pulmonary tuberculosis
X线计算机体层摄影术
animal models
pathology
X-ray computed tomography
动物模型
肺结核
病理学
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ISSN1008-6358
DOI10.12025/j.issn.1008-6358.2017.20170599

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Summary:目的：探讨micro-CT对急性肺结核小鼠动态演变过程的监测价值。方法：用滴鼻法建立急性感染C57BL/6J小鼠肺结核模型,设为实验组,同时设立对照组。两组分别在实验组滴鼻后第1、4、8、12周进行micro-CT扫描,并与病理学检查结果对比。结果：实验组共40只小鼠,死亡9只,3只肺内阴性,余28只小鼠分为4个亚组（分别7、6、8、7只）。对照组4亚组,每组5只。实验组小鼠感染第1周,71.4%表现为磨玻璃密度影（GGO）,57.1%表现为支气管充气征,对应病理表现为肺泡腔渗出和肺泡间隔增宽;感染第4周,micro-CT主要表现为GGO和小灶性实变（66.7%）,对应病理表现为肺泡腔渗出和实变,可见类上皮细胞、多核巨细胞和泡沫细胞;感染第8周,结节、支气管充气征和实变3种表现比例相当,病理对应实变和结核小结;感染第12周,结节出现比例最高（57.1%）,对应病理表现为结核肉芽肿形成。结论：急性小鼠肺结核模型的肺部micro-CT及病理动态演变基本一致,可用于模拟人类肺结核感染过程,评估疾病进程。
Bibliography:31-1794/R
Objective：To study the value of micro-CT in monitoring the dynamic evolution of acute pulmonary tuberculosis in mice.Methods：Establish the murine tuberculosis model of C57BL/6J in intranasal as the experiment group.The control group was set at the SPF level.Micro-CT scans were undertaken 1,4,8,12 weeks later,respectively.And then the mice were sacrificed and the lung tissue was taken for pathological analysis.Results：In the experiment group,nine mice died and three were negative.So,totally 28 mice were enrolled.There were 7,6,8,7 mice in each subgroup,respectively.One week after infection,ground glass opacity（GGO）was 71.4% and consolidation was 57.1% in the experiment group.Corresponding pathology showed alveolar exudate and alveolar septum widened.Four weeks after infection,the imaging findings included GGO and patchy opacities （in 66.7%）in the experiment group.Microscopically,alveolar effusion and consolidation,with epithelioid cells,multinucleated giant cells and foam cells were found.Eight weeks
ISSN:1008-6358
DOI:10.12025/j.issn.1008-6358.2017.20170599