部分杧果种质资源遗传多样性的SRAP和ISSR分析

利用SRAP和ISSR分子标记,对20份杧果种质资源进行遗传亲缘关系分析,为杧果种质资源的鉴定和杂交育种亲本选配提供参考依据。结果显示,在SRAP分析中,从80对引物中选出12对引物组合共扩增出224条谱带,每对引物组合扩增谱带数在13～21条,平均为18.7条,其中多态性谱带为194条,平均多态性谱带为16.2条,多态性比率为86.61%,材料间相似遗传系数变化范围为0.54～0.94;在ISSR分析中,从100条引物中选出10条引物共扩增出179条谱带,每条引物扩增谱带数在11～20条,平均为17.9条,其中多态性谱带为153条,平均多态性谱带为15.3,多态性比率为85.35%,材料间相...

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Published in西南农业学报 Vol. 29; no. 9; pp. 2045 - 2051
Main Author 张宇 黄国弟 黄强
Format Journal Article
LanguageChinese
Published 广西壮族自治区亚热带作物研究所,广西南宁,530001 2016
Subjects
SRAP
TRAP
杧果
遗传多样性
genetic diversity
遗传多样性
SRAP
杧果
TRAP
ISSR
mango
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Summary:利用SRAP和ISSR分子标记,对20份杧果种质资源进行遗传亲缘关系分析,为杧果种质资源的鉴定和杂交育种亲本选配提供参考依据。结果显示,在SRAP分析中,从80对引物中选出12对引物组合共扩增出224条谱带,每对引物组合扩增谱带数在13～21条,平均为18.7条,其中多态性谱带为194条,平均多态性谱带为16.2条,多态性比率为86.61%,材料间相似遗传系数变化范围为0.54～0.94;在ISSR分析中,从100条引物中选出10条引物共扩增出179条谱带,每条引物扩增谱带数在11～20条,平均为17.9条,其中多态性谱带为153条,平均多态性谱带为15.3,多态性比率为85.35%,材料间相似遗传系数变化范围为0.58～0.90。SRAP和ISSR标记分别在相似系数0.624和0.665水平上,均可将20份杧果种质资源分成4大类群,SRAP和ISSR标记都能将供试材料完全区分开来,聚类结果具有很高的一致性,均适用于杧果材料的遗传多样性分析,可用于杧果遗传亲缘关系的研究。
Bibliography:ZHANG Yu,HUANG Guo-di,HUANG Qiang （ Guangxi Subtropical Crops Research Institute, Guangxi Nanning 530001, China）
mango; genetic diversity; SRAP; ISSR
51-1213/S
The genetic variation and relationship of 20 mango germplasms were analyzed by SRAP and ISSR molecular markers in order to provide references for the identification of mango germplasm resources and breeding selection of parents. In SRAP analysis,from 80 pairs of primers,12 pairs of primers were selected and 224 bands were amplified. Each primer combination amplified polymorphism belt in 13- 21,with the average of 18. 7. There were 194 polymorphic bands,with the average of 16. 2. Polymorphism ratio was 86. 61 %. The genetic similarity coefficient of materials was 0. 54- 0. 94. In the ISSR analysis,from 100 primers,10 primers amplified 179 bands. Each primer amplified bands from 11 to 20,with an average of 17. 9,of which 153 were polymorphic bands. The average polymorphism was 15. 3 and polymorphic ratio was 85. 35 %. The genetic similarity coefficient of m
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2016.09.007