基于SSR标记的李种质资源遗传多样性研究

利用13对SSR引物对47份李种质资源进行了遗传多样性分析,结果表明：13对引物在47个样品中的平均等位基因数（Na）为10.846,有效等位基因数（Ne）平均值为4.806,观察杂合度（Ho）平均值为0.746,平均期望杂合度（He）为0.774,Shannon多样性指数（I）平均值为1.817,说明供试的李种质资源具有较高的遗传多样性。基于Nei距离的Neighbor-Joining聚类分析结果表明：47个李样品被分为3个组,同时能够清楚地鉴别出同物异名的种质资源。基于贝叶斯方法的STRUCTURE聚类分析进一步证实了这一结果。表明日本和美国的李品种与我国南方李品种群亲缘关系较近。...

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Bibliographic Details
Published in江西农业学报 Vol. 29; no. 2; pp. 33 - 39
Main Author 孙萍 林贤锐 沈建生
Format Journal Article
LanguageChinese
Published 浙江省金华市农业科学研究院,浙江 金华,321000 2017
Subjects
SSR标记
李
种质资源
遗传多样性
遗传结构
Plum
遗传多样性
Genetic structure
种质资源
Genetic diversity
SSR marker
Germplasm resource
李
SSR标记
遗传结构
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ISSN1001-8581
DOI10.19386/j.cnki.jxnyxb.2017.02.07

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Summary:利用13对SSR引物对47份李种质资源进行了遗传多样性分析,结果表明：13对引物在47个样品中的平均等位基因数（Na）为10.846,有效等位基因数（Ne）平均值为4.806,观察杂合度（Ho）平均值为0.746,平均期望杂合度（He）为0.774,Shannon多样性指数（I）平均值为1.817,说明供试的李种质资源具有较高的遗传多样性。基于Nei距离的Neighbor-Joining聚类分析结果表明：47个李样品被分为3个组,同时能够清楚地鉴别出同物异名的种质资源。基于贝叶斯方法的STRUCTURE聚类分析进一步证实了这一结果。表明日本和美国的李品种与我国南方李品种群亲缘关系较近。
Bibliography:Thirteen pairs of SSR（Simple Sequence Repeats） primers were used to evaluate the genetic diversity of 47 accessions of plum germplasm resources. The results showed that the mean number of polymorphic alleles（Na） was 10.846,the number of effective alleles（Ne） per locus was 4.806,the average observed heterozygosity（Ho） was 0.746,the average expected heterozygosity（He） was 0.774,and the mean Shannon＇s diversity index（I） was 1.817,suggesting the tested plum germplasm resources had a high genetic diversity. Neighbor-Joining clustering analysis based on Nei＇s distance indicated that 47 plum samples were divided into 3 groups,and the synonyms of these germplasm resources could be identified clearly,which was similar to the results of STRUCTURE clustering analysis based on Bayesian method. It was concluded that the plum germplasm resources from Japan and USA had a closer phylogenetic relationship with those from the Southern China.
36-1124/S
Plum; Germplasm resource; SSR marker; Genetic diversity; Genetic structure
SUN
ISSN:1001-8581
DOI:10.19386/j.cnki.jxnyxb.2017.02.07