哈茨木霉gz-2菌株在土壤中的定殖规律研究

为了明确拮抗木霉在土壤中的定殖动态,采用稀释分离法,测试了木霉gz-2菌株随时间推移在土壤中垂直及水平方向的数量变化情况。结果表明,在垂直方向上,26 cm深度以内,gz-2菌株的定殖数量总体呈先上升后下降的趋势,深度越深,其数量达到峰值的时间越短;深度超过28 cm,其数量不再上升;56 d内,gz-2菌株的最大定殖深度为40 cm。在水平方向上,gz-2菌株呈阶梯式扩散模式,其定殖数量总体呈先上升后下降趋势,并在接种后第35 d达到峰值;63 d内,最大定殖半径为24 cm。土壤含水量和p H对gz-2菌株在土壤中定殖数量的影响测试结果表明,当土壤含水量为25%,pH为7.8时,该菌株在土...

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Published in西南农业学报 Vol. 29; no. 1; pp. 138 - 142
Main Author 杜婵娟 付岗 潘连富 叶云峰 黄志君
Format Journal Article
LanguageChinese
Published 广西农业科学院微生物研究所,广西南宁,530007%广西农业科学院园艺研究所,广西南宁,530007%横县科学技术情报研究所,广西南宁,530300 2016
Subjects
pH
土壤含水量
木霉
空间定殖
Trichoderma
土壤含水量
Soil moisture
pH
木霉
空间定殖
Colonization
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Summary:为了明确拮抗木霉在土壤中的定殖动态,采用稀释分离法,测试了木霉gz-2菌株随时间推移在土壤中垂直及水平方向的数量变化情况。结果表明,在垂直方向上,26 cm深度以内,gz-2菌株的定殖数量总体呈先上升后下降的趋势,深度越深,其数量达到峰值的时间越短;深度超过28 cm,其数量不再上升;56 d内,gz-2菌株的最大定殖深度为40 cm。在水平方向上,gz-2菌株呈阶梯式扩散模式,其定殖数量总体呈先上升后下降趋势,并在接种后第35 d达到峰值;63 d内,最大定殖半径为24 cm。土壤含水量和p H对gz-2菌株在土壤中定殖数量的影响测试结果表明,当土壤含水量为25%,pH为7.8时,该菌株在土壤中的数量达到最大值,为3.38×107cfu/g。
Bibliography:Trichoderma; Colonization; Soil moisture; pH
51-1213/S
In order to determine the colonization dynamics of Trichoderma in soil,the quantity change of T. harzianum strain gz-2 was determined by dilution isolation in vertical and horizontal direction of soil with time changed. The results showed that,in vertical direction of soil,the colonization amount of strain gz-2 above the depth of 26 cm tended to increase first and then decreased,the amount of strain gz-2 reaching peak was the deeper the earlier. When the depth was over 28 cm,the amount of strain gz-2 increased no longer. The maximum depth of colonization was 40 cm in 56 days. Strain gz-2 was spread with stepwise model in horizontal direction of soil,its colonization amount tended to increase first and then decreased,and reached the peak at 35 days. The maximum radius of colonization was 24 cm in 63 days. The effect of the soil moisture and pH on colonization amount of strain gz-2 was determined. The results showed that,when the soil moisture was 25 %and pH
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2016.01.027